Skip to main content
Log in

Impact of the preparation technique on DNA content measured by image analysis in early stage human testis cancer

  • Published:
International Urology and Nephrology Aims and scope Submit manuscript

This is a preview of subscription content, log in via an institution to check access.

Access this article

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

References

  1. Baldetorp, B., Ferno, M., Fallenius, A., Fallenius-Vecchi, G., Idvall, I., Olsson, H., Sigurdson, H., Akerman, M., Killander, D.: Image cytometric DNA analysis in human breast cancer analysis may add prognostic information in diploid cases with low S-phase fraction by flow cytometry.Cytometry, 13, 577 (1992).

    Article  PubMed  CAS  Google Scholar 

  2. Becker, R. L., Mikel, U. V.: Interrelation of formalin fixation, chromatin compactness and DNA values as measured by flow and image analysis.Anal. Quant. Cytol. Histol., 12, 333 (1990).

    PubMed  Google Scholar 

  3. Einhorn, L. H.: Treatment of testicular cancer.J. Clin. Oncol., 8, 1777 (1990).

    PubMed  CAS  Google Scholar 

  4. Feichter, G. E., Goerttler, K.: Pitfalls in the preparation of nuclear suspensions from paraffin-embedded tissue for flow cytometry.Cytometry, 7, 616 (1986).

    Article  PubMed  CAS  Google Scholar 

  5. Feulgen, R., Rosenbeck, H.: Mikroskopisch-chemischer Nachweis einer Nukleinsäure vom Typus der Thymonukleinsäure und die darauf beruhende elektive Färbung von Zellkernen in mikroskopischen Präparaten.Zschr. Physiol. Chem., 135, 203 (1924).

    CAS  Google Scholar 

  6. Giroud, F., Montmassan, M. P.: Reevaluation of optimal Feulgen reaction for automated cytology. Influences of fixatives.Anal. Quant. Cytol. Histol., 11, 87 (1989).

    PubMed  CAS  Google Scholar 

  7. Hedley, D. W., Friedlander, M. L., Taylor, I. W., Rugg, C. A., Musgrove, E. A.: Method for analysis of cellular DNA content of paraffin-embedded pathological material using flow cytometry.J. Histochem. Cytochem., 31 (11), 1333 (1983).

    PubMed  CAS  Google Scholar 

  8. Hedley, D. W.: Flow cytometry using paraffin-embedded tissue: Five years on.Cytometry, 10, 229 (1989).

    Article  PubMed  CAS  Google Scholar 

  9. Hedley, D. W.: DNA analysis from paraffin-embedded blocks.Cell Biology, 33, 139 (1990).

    Article  CAS  Google Scholar 

  10. Launoit, Y., Kiss, R., Danguy, A.: Influence of smear preparation and fixatives on the DNA ploidy and the morphonuclear features of the MXT mammary tumor and normal tissues in the mouse.Cytometry, 11, 691 (1990).

    Article  PubMed  Google Scholar 

  11. Mikuz, G., Hofstaedter, F., Delgado, R.: Extraction of cells from paraffin-embedded sections for single-cell DNA cytometry.Analyt. Quant. Cytol., 7, 343 (1985).

    CAS  Google Scholar 

  12. Pizzocaro, Z., Nicolai, N., Salrioni, R., Pira, L., Faustini, M., Fanoni, F., Milani, A.: Comparison between clinical and pathological staging in low stage nonseminomatous germ cell testicular tumors.J. Urol., 148, 76 (1992).

    PubMed  CAS  Google Scholar 

  13. Van Driel-Kulker, A. M. J., Meseker, W. E., van Velzen, I., Tanke, H. J., Feichtinger, J., Ploem, J. S.: Preparation of monolayer smears from paraffin-embedded tissue for image cytometry.Cytometry, 6, 268 (1985).

    Article  PubMed  Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Rights and permissions

Reprints and permissions

About this article

Cite this article

De Riese, C., Crabtree, W.N., De Riese, W. et al. Impact of the preparation technique on DNA content measured by image analysis in early stage human testis cancer. International Urology and Nephrology 27, 603–613 (1995). https://doi.org/10.1007/BF02564748

Download citation

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF02564748

Keywords

Navigation