Advertisement

Journal of the American Oil Chemists’ Society

, Volume 70, Issue 12, pp 1289–1291 | Cite as

Improved high-performance liquid chromatography of vitamin E vitamers on normal-phase columns

  • Tai-Sun Shin
  • J. Samuel Godber
Short Communication

Abstract

A method is described for improving column stability and reproducibility for analysis of vitamin E vitamers on normal-phase high-performance liquid chromatography columns with isooctane/ethyl acetate/acetic acid/2,2-dimethoxypropane (98.5:0.9:0.85:0.1) mobile phase. Acetic acid in the mobile phase reduced retention times of vitamin E vitamer and increased column stability. A small concentration of 2,2-dimethoxypropane, which reacts with water to form acetone and methanol, reduced the need for column regeneration and stabilized retention times.

Key words

2,2-Dimethoxypropane normal-phase column rice bran vitamin E vitamer 

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. 1.
    Piironen, V., E.-L. Syväoja, P. Varo, K. Salminen and P. Koivistoninen,Cereal Chem. 63(2):78 (1986).Google Scholar
  2. 2.
    Meijboom, P.W., and G.A. Jongenotter,J. Am. Oil Chem. Soc. 56:33 (1979).Google Scholar
  3. 3.
    Whittle, K.J., P.J. Dunphy and J.F. Pennock,Biochem. J. 100:138 (1966).Google Scholar
  4. 4.
    Tan, B., and L. Brzuskiewicz,Anal. Biochem. 180:368 (1989).CrossRefGoogle Scholar
  5. 5.
    Lang, J.K., M. Schillaci and B. Irvin, inModern Chromatographic Analysis of the Vitamins, 2nd edn., edited by A.P. De Leenheer, |W.E. Lambert and H.J. Nelis, Marcel Dekker, Inc., New York, 1992, p. 153.Google Scholar
  6. 6.
    Nelis, H.J., V.O.R.C. DeBevere and A.P. De Leenheer, inModern Chromatographic Analysis of the Vitamins, edited by A.P. De Leenheer, W.E. Lambert and M.G.M. De Ruyter, Marcel Dekker, Inc., New York, 1985, p. 129.Google Scholar
  7. 7.
    Critchfield, F.E., and E.T. Bishop,Anal. Chem. 33:1034 (1961).CrossRefGoogle Scholar
  8. 8.
    Erley, D.S., Ibid.:1564 (1957).CrossRefGoogle Scholar
  9. 9.
    Adams, R.F.,J. Chromatogr. 95:189 (1974).CrossRefGoogle Scholar
  10. 10.
    Bredeweg, R.A., L.D. Rothman and C.D. Pfeiffer,Anal. Chem. 51:2061 (1979).CrossRefGoogle Scholar

Copyright information

© American Oil Chemists’ Society 1993

Authors and Affiliations

  • Tai-Sun Shin
    • 1
  • J. Samuel Godber
    • 1
  1. 1.Department of Food Science, Louisiana Agricultural Experimental StationLouisiana State University Agricultural CenterBaton Rouge

Personalised recommendations