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Molecular species composition of phosphatidylcholine fromCrypthecodinium cohnii in relation to growth temperature

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Lipids

Abstract

The molecular species composition was determined for phosphatidylcholine (PC) isolated from the marine dinoflagellateCrypthecodinium cohnii grown at three different temperatures. At all three temperatures the didocosahexaenoyl species comprised about 25% of the PC with 14∶0/22∶6 and 16∶0/22∶6 also being of major importance; these three species comprised 75–82% of the total. Another 20 species were identified, including several short chain disaturated species. Only small differences in the composition of PC were found in response to growth at 16, 23 and 27°C. On dropping the growth temperature from 27°C to 16°C the largest changes were a decrease of 8.9% in saturated/saturated species and an increase of 5.3% in saturated/PUFA species; the 22∶6/22∶6 content only increased slightly (by 1.9% to 25.4%). This unusual molecular species composition is discussed.

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Abbreviations

BHT:

butylated hydroxytoluene

HPLC:

high performance liquid chromatography

HPTLC:

high performance thin-layer chromatography

PC:

phosphatidylcholine

PE:

phosphatidylethanolamine

PS:

phosphatidylserine

PUFA:

polyunsaturated fatty acid

TLC:

thin-layer chromatography

molecular species:

e.g., 14∶0/22∶6, 1-myristoyl-2-docosahexaenoyl-sn-glycero-3-phosphocholine

References

  1. Harrington, G.W., and Holz, G.G. (1968)Biochim. Biophys. Acta 164, 137–139.

    PubMed  CAS  Google Scholar 

  2. Beach, D.H., and Holz, G.G. (1973)Biochim. Biophys. Acta 316, 56–65.

    PubMed  CAS  Google Scholar 

  3. Henderson, R.J., Leftley, J.W., and Sargent, J.R. (1988)Phytochemistry 27, 1679–1683.

    Article  CAS  Google Scholar 

  4. Tuttle, R.C., and Loeblich, A.R. (1975)Phycologia 14, 1–8.

    Google Scholar 

  5. Folch, J., Lees, M., and Sloane Stanley, G.H. (1957)J. Biol. Chem. 226, 497–509.

    PubMed  CAS  Google Scholar 

  6. Vitiello, F., and Zanetta, J.-P. (1978)J. Chromatogr. 166, 637–640.

    Article  PubMed  CAS  Google Scholar 

  7. Parsons, J.G., and Patton, S. (1967)J. Lipid Res. 8, 696–698.

    PubMed  CAS  Google Scholar 

  8. Takamura, H., Narita, H., Urade, R., and Kito, M. (1986)Lipids 21, 356–361.

    Article  PubMed  CAS  Google Scholar 

  9. Bell, M.V. (1989)Lipids 24, 585–588.

    Article  CAS  Google Scholar 

  10. Takahashi, K., Hirano, T., Takama, K., and Zama, K. (1982)Bull. Japan. Soc. Sci. Fish. 48, 1803–1814.

    CAS  Google Scholar 

  11. Robinson, M., Blank, M.L., and Snyder, F. (1986)Arch. Biochem. Biophys. 250, 271–279.

    Article  PubMed  CAS  Google Scholar 

  12. Blank, M.L., Cress, E.A., Robinson, M., and Snyder, F. (1985)Biochim. Biophys. Acta 833, 366–371.

    PubMed  CAS  Google Scholar 

  13. Aveldano, M.I., and Bazan, N.G. (1983)J. Lipid Res. 24, 620–627.

    PubMed  CAS  Google Scholar 

  14. Bell, M.V., and Tocher, D.R. (1989)Biochem. J. 264, 909–915.

    PubMed  CAS  Google Scholar 

  15. Heinz, E. (1977) inLipids and Lipid Polymers in Higher Plants, pp. 102–120, Springer-Verlag, Berlin and New York.

    Google Scholar 

  16. Tocher, D.r., and Harvie, D.G. (1988)Fish Physiol. Biochem. 5, 229–239.

    Article  CAS  Google Scholar 

  17. Applegate, K.R., and Glomset, J.A. (1986)J. Lipid Res. 27, 658–680.

    PubMed  CAS  Google Scholar 

  18. Hazel, J.R., and Landrey, S.R. (1988)Am. J. Physiol. 255, R628-R634.

    PubMed  CAS  Google Scholar 

  19. Harel, Z., and Djerassi, C. (1980)Lipids 15, 694–696.

    Article  PubMed  CAS  Google Scholar 

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Authors and Affiliations

  1. NERC Unit of Aquatic Biochemistry, School of Molecular and Biological Sciences, University of Stirling, FK9 4LA, Stirling, UK

    Michael V. Bell & R. James Henderson

Authors
  1. Michael V. Bell
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  2. R. James Henderson
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Bell, M.V., Henderson, R.J. Molecular species composition of phosphatidylcholine fromCrypthecodinium cohnii in relation to growth temperature. Lipids 25, 115–118 (1990). https://doi.org/10.1007/BF02562215

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  • DOI: https://doi.org/10.1007/BF02562215

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