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Lability of red blood cell membranes to lipid peroxidation: Application to humans fed polyunsaturated lipids

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Lipids

Abstract

Red blood cell membranes (RBCM) were used to estimate human red blood cell lability to lipid peroxidationin vitro. RBCM were prepared from blood collected from humans fed diets with either 3 or 15% polyunsaturated fatty acids for 80 days. RBCM were isolated by centrifugation, and oxidative stress was induced byin vitro incubation with 0.1 or 0.5 mM tert-butyl hydroperoxide (t-BOOH) in the presence of 0.5 mg added hemoglobin. Lipid Peroxidation was evaluated by measurement of thiobarbituric acid-reactive substances (TBARS). Lipid peroxidation correlated with the protein content of RBCM in both noninduced and t-BOOH-induced lipid peroxidation systems. TBARS production was dependent on the amount of t-BOOH added to the RBCM. The production of TBARS by RBCM incubated with 0.5 mM t-BOOH was correlated with the arachidonic acid content in the red blood cells (RBC) from which RBCM were prepared. The methodology developed was useful for comparative estimations of the lability of RBCM to lipid peroxidation.

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Abbreviations

GC:

gas chromatography

PUFA:

polyunsaturated fatty acids

RBC:

red blood cells

RBCM:

red blood cell membranes

TBARS:

thiobarbituric acid-reactive membranes

t-BOOH:

tert-butyl hydroperoxide

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Author information

Author notes

  1. Cesar G. Fraga

    Present address: Catedra de Fisicoquímica, Facultad de Farmacia y Bioquímica, Universidad de Buenos Aires, Junin 956 1113, Buenos Aires, Argentina

  2. Daniel Chiu

    Present address: Center for Advanced Medical Technology, San Francisco State University, 94132, San Francisco, CA

Authors and Affiliations

  1. Department of Food Science and Technology, University of California, 1480 Chemistry Annex, 95616, Davis, CA

    Cesar G. Fraga, A. L. Tappel & Brian E. Leibovitz

  2. Children's Hospital Medical Center, Oakland, California

    Franz Kuypers & Daniel Chiu

  3. Western Human Nutrition Research Center, Presidio of San Francisco, California

    James M. Iacono & Darshan S. Kelley

Authors
  1. Cesar G. Fraga
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  2. A. L. Tappel
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  3. Brian E. Leibovitz
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  4. Franz Kuypers
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  5. Daniel Chiu
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  6. James M. Iacono
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  7. Darshan S. Kelley
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Fraga, C.G., Tappel, A.L., Leibovitz, B.E. et al. Lability of red blood cell membranes to lipid peroxidation: Application to humans fed polyunsaturated lipids. Lipids 25, 111–114 (1990). https://doi.org/10.1007/BF02562214

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  • DOI: https://doi.org/10.1007/BF02562214

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