Skip to main content
SpringerLink
Log in

Synergistic activation of CTP:phosphocholine cytidylyltransferase by phosphatidylethanolamine and oleic acid

  • Published:
Lipids

Abstract

CTP:phosphocholine cytidylyltransferase present in rat liver cytosol was activated almost 30-fold when assayed in the presence of liposomes containing 60 mole % dioleoyl phosphatidylethanolamine (DOPE). During the assay, some of the DOPE was degraded to lysoPE and oleic acid. Whereas cytidylyltransferase activity was not affected when assayed in the presence of liposomes containing lysoPE, liposomes containing oleic acid activated the enzyme. Activation by oleic acid could be eliminated by the addition of fatty acid-free bovine serum albumin (BSA) to the assay. When cytidylyltransferase activity was measured in the presence of both BSA and liposomes containing DOPE, enzyme activity was increased almost 20-fold, as compared with assays performed in the absence of added lipid. The 1.5-fold difference in cytidylyltransferase activity observed when cytosol was assayed with DOPE containing liposomes in the absence or presence of BSA (30-fold stimulation vs 20-fold stimulation) cannot be explained by the loss of activation attributable to oleic acid alone. Activation of the enzyme in the presence of liposomes containing DOPE and oleic acid is several-fold greater than the sum of the activations caused by the individual compounds. These data suggest that PE and oleic acid act synergistically in activating the cytidylyltranferase.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

Abbreviations

BSA:

bovine serum albumin

DOPC:

dioleoyl phosphatidylcholine

DOPE:

dioleoyl phosphatidylethanolamine

DPPE:

dipalmitoyl phosphatidylethanolamine

EDTA:

ethylene-diaminetetraacetic acid

PC:

phosphatidylcholine

PE:

phosphatidylethanolamine

References

  1. Pelech, S.L., and Vance, D.E. (1984)Biochim. Biophys. Acta 779, 217–251.

    PubMed  CAS  Google Scholar 

  2. Vance, J.E., and Vance, D.E. (1985)Can. J. Biochem. Cell Biol. 63, 870–881.

    Article  PubMed  CAS  Google Scholar 

  3. Rooney, S.A. (1985)Am. Rev. Respir. Dis. 131, 439–460.

    PubMed  CAS  Google Scholar 

  4. Vance, D.E. (1985) inBiochemistry of Lipids and Membranes, (Vance, D.E., and Vance, J.E., eds.), pp. 242–270, Benjamin/Cummings, Menlo Park, CA.

    Google Scholar 

  5. Wilgram, G.F., and Kennedy, E.P. (1963)J. Biol. Chem. 238, 2615–2619.

    PubMed  CAS  Google Scholar 

  6. Nemat-Gorgani, M., and Wilson, J.E. (1980)Curr. Top. Cell. Regul. 16, 45–54.

    PubMed  CAS  Google Scholar 

  7. Sleight, R.G., and Kent, C. (1983)J. Biol. Chem. 258, 831–835.

    PubMed  CAS  Google Scholar 

  8. Choy, P.C., and Vance, D.E. (1978)J. Biol. Chem. 253, 5163–5167.

    PubMed  CAS  Google Scholar 

  9. Choy, P.C., Farren, S.B., and Vance, D.E. (1979)Can. J. Biochem. 57, 605–612.

    Article  PubMed  CAS  Google Scholar 

  10. Feldman, D.A., Kovac, C.R., Dranginis, P.L., and Weinhold, P.A. (1978)J. Biol. Chem. 253, 4980–4986.

    PubMed  CAS  Google Scholar 

  11. Wright, P.S., Morand, J.N., and Kent, C. (1985)J. Biol. Chem. 260, 7919–7926.

    PubMed  CAS  Google Scholar 

  12. Sleight, R.G., and Kent, C. (1980)J. Biol. Chem. 255, 10644–10650.

    PubMed  CAS  Google Scholar 

  13. Pelech, S.L., Pritchard, P.H., Brindley, D.N., and Vance, D.E. (1983)J. Biol. Chem. 258, 6782–6788.

    PubMed  CAS  Google Scholar 

  14. Weinhold, P.A., Feldman, D.A., Quade, M.M., Miller, J.C., and Brooks, R.S. (1981)Biochim. Biophys. Acta 665, 134–144.

    PubMed  CAS  Google Scholar 

  15. Pelech, S.L., Cook, H.W., Paddon, H.B., and Vance, D.E. (1984)Biochim. Biophys. Acta 795, 433–440.

    PubMed  CAS  Google Scholar 

  16. Sleight, R.G., and Kent, C. (1983)J. Biol. Chem. 258, 836–839.

    PubMed  CAS  Google Scholar 

  17. Mansbach, C.M., and Arnold, A. (1986)Biochim. Biophys. Acta 875, 516–524.

    PubMed  CAS  Google Scholar 

  18. Chu, A.J., and Rooney, S.A. (1985)Biochim. Biophys. Acta 835, 132–140.

    PubMed  CAS  Google Scholar 

  19. Weinhold, P.A., Rousifer, M.E., and Feldman, D.A. (1986)J. Biol. Chem. 261, 5104–5110.

    PubMed  CAS  Google Scholar 

  20. Fiscus, W.G., and Schneider, W.C., (1966)J. Biol. Chem. 241, 3324–3330.

    PubMed  CAS  Google Scholar 

  21. Comfurius, P., and Zwaal, R.F.A. (1977)Biochim. Biophys. Acta 488, 36–42.

    PubMed  CAS  Google Scholar 

  22. Papahadjopoulos, D., Jacobson, K., Nir, S., and Isac, T. (1973)Biochim. Biophys. Acta 311, 330–348.

    Article  PubMed  CAS  Google Scholar 

  23. Cullis, P.R., de Kruijff, B., Hope, M.J., Verkleij, A.J., Nayar, R., Farren, S.B., Tilcock, C.P.S., Madden, T.D., and Bally, M.B. (1983) inMembrane Fluidity in Biology (Aloia, R.C., ed.) pp. 39–81, Academic Press, New York.

    Google Scholar 

  24. Tilcock, C.P.S., Bally, M.B., Farren, S.B., and Cullis, P.R. (1982)Biochemistry 21, 4596–4601.

    Article  PubMed  CAS  Google Scholar 

  25. Bligh, E.G., and Dyer, W.J. (1959)Can. J. Biochem. Physiol. 37, 911–917.

    PubMed  CAS  Google Scholar 

  26. Rodriguez de Turco, E.B., and Aveldano de Caldironi, M.I. (1980)Anal. Biochem. 104, 62–69.

    Article  PubMed  CAS  Google Scholar 

  27. Lowry, O.H., Rosebrough, N.J., Farr, A.L., and Randall, R.J. (1957)J. Biol. Chem. 193, 265–275.

    Google Scholar 

  28. Rouser, G., Fleischer, S., and Yamamoto, A. (1970)Lipids 5, 494–496.

    Article  PubMed  CAS  Google Scholar 

  29. Feldman, D.A., Brubaker, P.G., and Weinhold, P.A. (1981)Biochim. Biophys. Acta 665, 53–59.

    PubMed  CAS  Google Scholar 

  30. Weinhold, P.A., Rounsifer, M.E., Williams, S.E., Brubaker, P.G., and Feldman, D.A. (1984)J. Biol. Chem. 259, 10315–10321.

    PubMed  CAS  Google Scholar 

  31. Whitlon, D.S., Anderson, K.E., and Mueller, G.C. (1985)Biochim. Biophys. Acta 835, 369–377.

    PubMed  CAS  Google Scholar 

  32. Feldman, D.A., and Weinhold, P.A. (1987)J. Biol. Chem. 262, 9075–9081.

    PubMed  CAS  Google Scholar 

  33. Feldman, D.A., Dietrich, J.W., and Weinhold, P.A. (1980)Biochim. Biophys. Acta 620, 603–611.

    PubMed  CAS  Google Scholar 

  34. Pelch, S.L., and Vance, D.E. (1982)J. Biol. Chem. 257, 14198–14202.

    Google Scholar 

  35. Vance, D.E., Trip, E.M., and Paddon, H.B. (1980)J. Biol. Chem. 255, 1064–1069.

    PubMed  CAS  Google Scholar 

  36. Vigo, C., and Vance, D.E. (1981)Biochem. J. 200, 321–326.

    PubMed  CAS  Google Scholar 

  37. Tijburg, L.B.M., Houweling, M., Geelen, M.J.H., and van Golde, L.M.G. (1988)Biochim. Biophys. Acta 959, 1–8.

    PubMed  CAS  Google Scholar 

  38. Feldman, D.A., Rounsifer, M.E., and Weinhold, P.A. (1985)Biochim. Biophys. Acta 429, 429–437.

    Google Scholar 

  39. Cornell, R., and Vance, D.E. (1987)Biochim. Biophys. Acta 919, 37–48.

    PubMed  CAS  Google Scholar 

  40. Op den Kamp, J.A.F. (1979)Annu. Rev. Biochem. 48, 47–71.

    Article  PubMed  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of Molecular Genetics, Biochemistry and Microbiology, University of Cincinnati College of Medicine, 231 Bethesda Avenue, 45267-O524, Cincinnati, Ohio

    Richard G. Sleight & Hong-Ngoc Thi Dao

Authors
  1. Richard G. Sleight
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Hong-Ngoc Thi Dao
    View author publications

    You can also search for this author in PubMed Google Scholar

About this article

Cite this article

Sleight, R.G., Thi Dao, HN. Synergistic activation of CTP:phosphocholine cytidylyltransferase by phosphatidylethanolamine and oleic acid. Lipids 25, 100–107 (1990). https://doi.org/10.1007/BF02562212

Download citation

  • Received:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF02562212

Keywords

Search

Navigation