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The Na+K+ATPase activity in cultured human fibroblasts with an elevated phospholipid triene: tetraene ratio

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Lipids

Abstract

Human skin fibroblasts were cultured at low density for 11 days in MCDB 110, 0.4% fetal bovine serum, a mitogen mixture, and were supplemented with 18∶2n−6 or 18∶1n−9 as a fatty acid-albumin complex. The cells cultured with the 18∶2n−6 supplement had a 20∶3n−9/20∶4n−6 ratio of 0.29±0.07; the 18∶1n−9 supplemented cells had a ratio of 1.51±0.27. There was less than 4% difference in total growth of the cell population under the two culture conditions. The cells supplemented with 18∶2n−9 had similar levels of protein/cell, K+/mg cell protein and functional Na+K+ATPase activity.

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Abbreviations

ANS:

8-Anilino-1-naphthalene sulfonate

Ci:

curie

EFA:

essential fatty acid

FAMEs:

fatty acid methyl esters

FBS:

fetal bovine serum

GLC:

gas-liquid chromatography

HEPES:

N-2(-hydroxyethyl)piperazine-N-2-ethanesulfonic acid

MECL:

modified equivalent chain length

Na+K+ATPase:

sodium ion activated ATPase (adenosinetriphosphatase)

PUFA:

polyunsaturated fatty acid

TLC:

thin-layer chromatography

UV:

ultraviolet

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Authors and Affiliations

  1. Cellular Nutrition and Metabolism Research Unit, Department of Nutritional Sciences, College of Biological Science, University of Guelph, N1G 2W1, Guelph, Ontario, Canada

    Soverin Karmiol & William J. Bettger

Authors
  1. Soverin Karmiol
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  2. William J. Bettger
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Karmiol, S., Bettger, W.J. The Na+K+ATPase activity in cultured human fibroblasts with an elevated phospholipid triene: tetraene ratio. Lipids 25, 73–77 (1990). https://doi.org/10.1007/BF02562207

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  • DOI: https://doi.org/10.1007/BF02562207

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