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Crystal dissolution of biological and ceramic apatites

Calcified Tissue International volume 45, pages 95–103 (1989)Cite this article

Summary

High resolution transmission electron microscopy (Hr TEM) studies on biological and synthetic calcium phosphate have provided information on the dissolution process at the crystal level. The purpose of this study was to investigate the dissolution of ceramic hydroxyapatite (HA) after implantation using Hr TEM. Recovered HA ceramic implanted in bony and nonbony sites in animals and in periodontal pockets in humans were used for the study. For comparison, sections of human fluorotic enamel with caries and sections of shark enameloid previously exposed to 0.1 HCl were similarly investigated. Hr TEM studies demonstrated that in both the biological and ceramic apatites, the lattice and atomic defects were the starting points in the dissolution process. However, significant differences in the process of dissolution were observed: (1) biological apatite crystals showed preferential core dissolution whereas ceramic apatite crystals showed nonspecific dissolution at the cores and at the surfaces; (2) the dissolution of biological apatites appeared to consistently extend along the crystal's c-axis whereas dissolution of the ceramic HA did not appear to be correlated with the crystal's c-axis. The observed differences in crystal dissolution between biological and ceramic apatites may be attributed to the following: (1) the unique crystal/protein interaction present with biological apatites but absent in ceramic HA; (2) differences in defect distribution between biological and ceramic apatites which are due to the differences in the original of these defects; and (3) the longer morphological c-axis of biological apatites compared with that of ceramic apatites. This study provided for the first time, information on the dissolution process of implanted ceramic HA crystals and suggests that the crystal defects resulting from the sintering processes during the preparation of ceramic HA affect itsin vivo degradation and performance.

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Affiliations

  1. Unite de Recherche sur les Tissus Calcifies, UFR Odontologie, U225 INSERM, Nantes, France

    G. Daculsi & D. Mitre

  2. New York University College of Dentistry, New York, New York, USA

    R. Z. LeGeros

Authors
  1. G. Daculsi
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  2. R. Z. LeGeros
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  3. D. Mitre
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Daculsi, G., LeGeros, R.Z. & Mitre, D. Crystal dissolution of biological and ceramic apatites. Calcif Tissue Int 45, 95–103 (1989). https://doi.org/10.1007/BF02561408

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  • DOI: https://doi.org/10.1007/BF02561408

Key words

  • Apatite
  • Calcium phosphate
  • Dissolution
  • Biomaterial
  • Ultrastructure