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Phagocytosis of bone collagen by osteoclasts in two cases of pycnodysostosis

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Summary

Electron microscopic examination of bone biopsies obtained from two patients suffering from pycnodysostosis revealed that osteoclasts contained (sometimes large) cytoplasmic vacuoles filled with bone collagen fibrils. These vacuoles stained positive for acid phosphatase activity, thereby suggesting that bone matrix had been phagocytosed and subsequently exposed to hydrolytic enzymes of the lysosomal apparatus. Collagen-containing vacuoles were not observed in osteoclasts of individuals not suffering from this disease.

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Authors and Affiliations

  1. Laboratory of Histology and Cell Biology, Faculty of Medicine, University of Amsterdam, Meibergdreef 15, 1105, AZ Amsterdam, The Netherlands

    Vincent Everts

  2. Department of Pediatrics, Academic Medical Centre, University of Amsterdam, Amsterdam, The Netherlands

    Daniel C. Aronson

  3. Section of Experimental Oral Biology, Department of Periodontology, Faculty of Dentistry, University of Amsterdam, Amsterdam, The Netherlands

    Wouter Beertsen

Authors
  1. Vincent Everts
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  2. Daniel C. Aronson
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  3. Wouter Beertsen
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Everts, V., Aronson, D.C. & Beertsen, W. Phagocytosis of bone collagen by osteoclasts in two cases of pycnodysostosis. Calcif Tissue Int 37, 25–31 (1985). https://doi.org/10.1007/BF02557674

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  • DOI: https://doi.org/10.1007/BF02557674

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