Skip to main content
SpringerLink
Log in

Nineteen new microsatellite DNA polymorphisms in pigtailed macaques (Macaca nemestrina)

  • Short Communication
  • Published:
Primates Aims and scope Submit manuscript

Abstract

Microsatellite loci known to be polymorphic in baboons (Papio hamadryas) and/or humans were tested in pigtailed macaques (Macaca nemestrina) from the Washington Regional Primate Research Center. Nineteen polymorphisms were identified in the macaques, with an average of 9.2 alleles per locus and an average heterozygosity of 0.76. Seven loci were analyzed using radiolabelled PCR primers and standard gel electrophoresis. Twelve loci were studied using fluorescently labelled primers and the Perkin-Elmer ABI 377 genotyping system. Of these 19 pigtailed macaque polymorphisms, 12 were used to perform paternity testing among captive animals. In a set of 15 infants, this panel of 12 genetic polymorphisms was sufficient to establish paternity in all cases. The number of alleles per locus in pigtailed macaques was compared with the number of alleles in a sample of baboons, and no significant correlation was observed. This indicates that population genetic processes such as genetic drift and recurrent mutation act rapidly enough on these loci to eliminate any relationship in levels of polymorphism across those two species. These 19 loci will be valuable for a range of genetic studies in pigtailed macaques, including paternity testing, analysis of population structure and differentiation among wild populations, and genetic linkage mapping.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

References

  • Altmann, J.;Alberts, S. C.;Haines, S. A.;Dubach, J.;Muruthi, P.;Coote, T.;Geffen, E.;Cheesman, D. J.;Mututua, R. S.;Saiyalel, S. N.;Wayne, R. K.;Lacy, R. C.;Bruford, M. W. 1996. Behavior predicts gene structure in a wild primate group.Proc. Natl. Acad. Sci. USA, 93: 5797–5801.

    Article  PubMed  CAS  Google Scholar 

  • Bosch, M. L.;Schmidt, A.;Agy, M. B.;Kimball, L. E.;Morton, W. R. 1997. Infection ofMacaca nemestrina neonates with HIV-1 via different routes of inoculation.AIDS, 11: 1555–1563.

    Article  PubMed  CAS  Google Scholar 

  • Charmley, P.;Keretan, E.;Snyder, K.;Clark, E. A.;Concannon, P. 1995. Relative size and evolution of the germline repertoire of T-cell receptor beta-chain gene segments in nonhuman primates.Genomics, 25: 150–156.

    Article  PubMed  CAS  Google Scholar 

  • Coote, T.;Bruford, M. W. 1996. Human microsatellites applicable for analysis of genetic variation in apes and Old World monkeys.J. Hered., 87: 406–410.

    PubMed  CAS  Google Scholar 

  • Disotell, T. D. 1996. The phylogeny of the Old World monkeys.Evol. Anthropol., 5: 18–24.

    Article  Google Scholar 

  • Don, R. H.;Cox, P. T.;Wainwright, B. J.;Baker, K.;Mattick, J. S. 1991. Touchdown PCR to circumvent spurious priming during gene amplification.Nucleic Acids Res., 19: 4008.

    Article  PubMed  CAS  Google Scholar 

  • Ellsworth, J. A.;Hoelzer, G. A. 1998. Characterization of microsatellite loci in a New World primate, the mantled howler monkey (Alouatta palliata).Mol. Ecol., 7: 657–658.

    Article  PubMed  CAS  Google Scholar 

  • Gaur, L. K.;Nepom, G. T. 1996. Ancestral major histocompatibility complex DRB genes beget conserved patterns of localized polymorphisms.Proc. Natl. Acad. Sci, USA, 93: 5380–5383.

    Article  PubMed  CAS  Google Scholar 

  • Gaur, L. K.;Pandarpurkar, M.;Anderson, J. 1998. DQA-DQB linkage in Old World monkeys.Tissue Antigens, 51: 367–373.

    Article  PubMed  CAS  Google Scholar 

  • Gust, D. A.;Gordon, T. P.;Gergits, W. F.;Casna, N. J.;Gould, K. G.;McClure, H. M. 1996. Male dominance rank and offspring-initiated affiliative behaviors were not predictors of paternity in a captive group of pigtail macaques (Macaca nemestrina).Primates, 37: 271–278.

    Article  Google Scholar 

  • Heath-Lange, S.;Ha, J. C.;Sackett, G. P. 1999. Behavioral measurement of temperament in male nursery-raised infant macaques and baboons.Amer. J. Primatol., 47: 43–50.

    Article  CAS  Google Scholar 

  • Hoelzer, G. A.;Melnick, D. J. 1996. Evolutionary relationships of the macaques. In:Evolution and Ecology of the Macaque Societies,Fa,J. E.;Lindburg,D. G. (eds.), Cambridge Univ. Press, Cambridge, pp. 3–19.

    Google Scholar 

  • Inoue, M.;Takenaka, O. 1993. Japanese macaque microsatellite PCR primers for paternity testing.Primates, 34: 37–45.

    Article  Google Scholar 

  • Joag, S. V.;Adany, I.;Li, Z.;Foresman, L.;Pinson, D. M.;Wang, C.;Stephens, E. B.;Raghavan, R.;Narayan, O. 1997. Animal model of mucosally transmitted human immunodeficiency virus type 1 disease: intravaginal and oral deposition of simian/human immunodeficiency virus in macaques results in systemic infection, elimination of CD4+cells, and AIDS.J. Virol., 71: 4016–4023.

    PubMed  CAS  Google Scholar 

  • Knapp, L. A.;Ha, J. C.;Sackett, G. P. 1996. Parental MHC antigen sharing and pregnancy wastage in captive pigtailed macaques.J. Reprod. Immunol., 32: 73–88.

    Article  PubMed  CAS  Google Scholar 

  • Morin, P. A.;Kanthaswamy, S.;Smith, D. G. 1997. Simple sequence repeat (SSR) polymorphisms for colony management and population genetics in rhesus macaques (Macaca mulatta).Amer. J. Primatol., 42: 199–213.

    Article  CAS  Google Scholar 

  • Morin, P. A.;Mahboubi, P.;Wedel, S.;Rogers, J. 1998. Rapid screening and comparison of human microsatellite markers in baboons: allele size is conserved, but allele number is not.Genomics, 53: 12–20.

    Article  PubMed  CAS  Google Scholar 

  • Morin, P. A.;Wallis, J.;Moore, J. J.;Woodruff, D. S. 1994. Paternity exclusion in a community of wild chimpanzees using hypervariable simple sequence repeats.Mol. Ecol., 3: 469–477.

    PubMed  CAS  Google Scholar 

  • Nozawa, K.;Shotake, T.;Kawamoto, Y.;Tanabe, Y. 1982. Population genetics of Japanese monkeys: II. Blood protein polymorphisms and population structure.Primates, 23: 252–271.

    Article  Google Scholar 

  • Ott, S. M.;O'Hanlan, M.;Lipkin, E. W.;Newell-Morris, L. 1997. Evaluation of vertebral volumetric vs areal bone mineral density during growth.Bone, 20: 553–556.

    Article  PubMed  CAS  Google Scholar 

  • Perelygin, A. A.;Kammerer, C. M.;Stowell, N. C.;Rogers, J. 1996. Conservation of human chromosome 18 in baboons (Papio hamadryas): a linkage map of eight human microsatellites.Cytogenet. Cell Genet., 75: 207–209.

    PubMed  CAS  Google Scholar 

  • Preuss, T. M.;Stepniewska, I.;Jain, N.;Kaas, J. H. 1997. Multiple divisions of macaque precentral motor cortex identified with neurofilament antibody SMI-32.Brain Res., 767: 148–153.

    Article  PubMed  CAS  Google Scholar 

  • Rausell, E.;Bickford, L.;Manger, P. R.;Woods, T. M.;Jones, E. G. 1998. Extensive divergence and convergence in the thalamocortical projection to monkey somatosensory cortex.J. Neurosci., 18: 4216–4232.

    PubMed  CAS  Google Scholar 

  • Rogers, J.;Hixson, J. E. 1997. Baboons as an animal model for genetic studies of common human disease.Amer. J. Human Genet., 61: 489–493.

    CAS  Google Scholar 

  • Rogers, J.;Witte, S. M.;Kammerer, C. M.;Hixson, J. E.;MacCluer, J. W. 1995. Linkage mapping inPapio baboons: conservation of a syntenic group of six markers on human chromosome 1.Genomics, 28: 251–254.

    Article  PubMed  CAS  Google Scholar 

  • Rosenblum, L. L.;Supriatna, J.;Melnick, D. J. 1997. Phylogeographic analysis of pigtail macaque populations (Macaca nemestrina) inferred from mitochondrial DNA.Amer. J. Phys. Anthropol., 104: 35–45.

    Article  CAS  Google Scholar 

  • Shimizu, K.;Takenaka, O. 1991. DNA polymorphisms in the epsilon- and gamma-globin gene regions in Asian macaques.Biochem. Genet., 29: 189–202.

    PubMed  CAS  Google Scholar 

  • Timchenko, L.;Monckton, D. G.;Caskey, C. T. 1995. Myotonic dystrophy: an unstable CTG repeat in a protein kinase gene.Sem. Cell Biol., 6: 13–19.

    Article  CAS  Google Scholar 

  • VandeBerg, J. L.;Williams-Blangero, S. 1997. Advantages and limitations of nonhuman primates as animal models in genetic research on complex diseases.J. Med. Primatol., 26: 113–119.

    PubMed  CAS  Google Scholar 

  • Williams-Blangero, S.;Vandeberg, J. L.;Blangero, J.;Konigsberg, L.;Dyke, B. 1990. Genetic differentiation between baboon subspecies: relevance for biomedical research.Amer. J. Primatol., 20: 67–81.

    Article  Google Scholar 

  • Witte, S. M.;Rogers, J. 1999. Microsatellite polymorphisms in Bolivian squirrel monkeys (Saimiri boliviensis).Amer. J. Primatol., 47: 75–84.

    Article  CAS  Google Scholar 

  • Worlein, J. M.;Sackett, G. P. 1997. Social development in nursery-reared pigtailed macaques (Macaca nemestrina).Amer. J. Primatol., 41: 23–35.

    Article  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of Genetics, Southwest Foundation for Biomedical Research, 7620 N.W. Loop 410, 78245, San Antonio, Texas, U. S. A.

    Shalini Nair & Jeffrey Rogers

  2. Washington Regional Primate Research Center, University of Washington, Seattle, Washington, U. S. A.

    James Ha

  3. Southwest Regional Primate Research Center, 78245, San Antonio, Texas, U. S. A.

    Jeffrey Rogers

Authors
  1. Shalini Nair
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. James Ha
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Jeffrey Rogers
    View author publications

    You can also search for this author in PubMed Google Scholar

About this article

Cite this article

Nair, S., Ha, J. & Rogers, J. Nineteen new microsatellite DNA polymorphisms in pigtailed macaques (Macaca nemestrina). Primates 41, 343–350 (2000). https://doi.org/10.1007/BF02557603

Download citation

  • Received:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF02557603

Key Words

Search

Navigation