Abstract
Marmosets normally produce dizygotic twins sharing placental blood vessels and exchanging bone marrow cells. Each individual is therefore likely to be a blood chimaera. To date, marmosets had only been DNA fingerprinted using blood samples and probes 33.6 and 33.15, resulting in highly similar fingerprints among litter mates and little variation between other individuals, thereby limiting this method's use for individual identification and parentage testing. In this study, novel probes were applied to detect greater polymorphism and to produce individual-specific DNA fingerprints. As expected, blood DNA profiles of twins and triplets were virtually identical, confirming chimaerism in this tissue and identifying litter mates. Furthermore, these profiles were sufficiently variable to distinguish between sibs from different litters and between all other individuals. To produce individual-specific DNA fingerprints, the use of DNA extracted from tissues poor in leukocytes was essential. The findings demonstrate that, despite extensive blood chimaerism, marmoset colonies can be effectively DNA fingerprinted for indicidual identification, zygosity testing, and relationship studies.
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Ardito, G.;Lamberti, L.;Bigatti, P.;Crovella, S.;Oberto, G. 1995. No correlation between chimaerism and fertility inCallithrix jacchus (Callitrichidae, Primates).Int. J. Anthropol., 10: 15–19.
Armour, J. A. L.;Wong, Z.;Wilson, V.;Royle, N. J.;Jeffreys, A. J. 1989. Sequences flanking the repeat arrays flanking the human minisatellites: association with dispersed repeat elements.Nucl. Acids Res., 13: 4925–4935.
Benirschke, K.;Anderson, J. M.;Brownhill, L. E. 1962. Marrow chimaerism in the marmosets.Science, 138: 513–515.
Carter, R. E.;Wetton, J. H.;Parkin, D. T. 1989. Improved genetic fingerprinting using RNA probes.Nucl. Acids Res., 17: 5867.
Dixson, A. F.;Anzenberger, G.;Monteiro da Cruz, M. A. O.;Patel, I.;Jeffreys, A. J. 1992. DNA fingerprinting of free-ranging groups of common marmosets (Callithrix jacchus jacchus) in NE Brazil. In.Paternity in Primates: Genetic Tests and Theories,Martin,R. D.;Dixson,A. F.;Wickings,E. J. (eds.), Karger, Basel, pp. 192–202.
Dixson, A. F.;Hastie, N.;Patel, I.;Jeffreys, A. J. 1988. DNA ‘fingerprinting’ of captive family groups of common marmosets (Callithrix jacchus).Folia Primatol., 51: 52–55.
Ellegren, H.;Hartman, G.;Johansson, M.;Andersson, L. 1993. Major histocompatibility complex monomorphism and low levels of DNA fingerprinting variability in a reintroduced and rapidly expanding population of beavers.Proc. Natl. Acad. Sci. USA, 90: 8150–8153.
Farber, C. M.;Georges, M.;de Bock, G.;Verhest, A.;Simon, P.;Verschraegen-Spae, M.;Vassart, G. 1989. Demonstration of spontaneous XX/XY chimaerism by DNA fingerprinting.Human Genet., 82: 197–198.
Faulkes, C. G.;Abbott, D. H.;O'Brien, H. P.;Lau, L.;Roy, M. R.;Wayne, R. K.;Bruford, M. W. 1997. Micro- and macro-geographical genetic structure of colonies of naked mole-rats (Heterocephalus glaber).Mol. Ecol., 6: 615–628.
Feinberg, A. P.;Vogelstein, B. 1983. A technique for radiolabelling DNA restriction endonuclease fragments to high specific activity.Anal. Biochem., 132: 6–13.
Ford, C. E.;Evans, E. P. 1977. Cytogenetic observations on XX/XY chimaeras and a reassessment of the evidence for germ cell chimaerism in heterosexual twin cattle and marmosets.J. Reprod. Fertil., 49: 25–33.
Gengozian, N.;Brewen, J. G.;Preston, R. J.;Batson, J. S. 1980. Presumptive evidence for the absence of functional germ cell chimaerism in the marmoset.J. Med. Primatol., 9: 9–27.
Gilgenkrantz, S.;Marchal, C.;Wendremaire, P.;Seger, M. 1981. Cytogenetic and antigenetic studies in a pair of twins: a normal boy and a trisomic 21 girl with chimera. In:Twin Research 3: Twin Biology and Multiple Pregnancy. A. R. Liss, New York, pp. 141–153.
Hampton, S. M. H. 1973. Germ cell chimerism in male marmosets.Amer. J. Phys. Anthropol., 38: 265–268.
Hill, A. V. S.;Jeffreys, A. J. 1985. Use of minisatellite DNA probes for determination of twin zygosity at birth.Lancet, ii: 1394–1395.
Hill, J. P. 1926. Demonstration of the embryologia varia (development ofHapale jacchus).J. Anatomy, 60: 486–487.
Jeffreys, A. J. 1987. Highly variable minisatellites and DNA fingerprints.Biochem. Soc. Trans., 15: 309–317.
Jeffreys, A. J.;Wilson, V.;Thein, S. L. 1985. Hypervariable minisatellite regions in human DNA.Nature, 314: 67–73.
Menotti-Raymond, M.;O'Brien, S. J. 1993. Dating the genetic bottleneck of the African cheetah.Proc. Natl. Acad. Sci. USA, 90: 3172–3176.
Pena, S. D. J.;Chakraborty, R.;Epplen, J. T.;Jeffreys, A. J. (eds.). 1993.DNA Fingerprinting: State of the Science. Birkhäuser Verlag, Basel.
Plante, Y.;Schmutz, S. M.;Lang, D. M.;Moker, J. S. 1992. Detection of leucochimaerism in bovine twins by DNA fingerprinting.Anim. Genet., 23: 295–302.
Signer, E. N.;Gu, F.;Jeffreys, A. J. 1996. A panel of VNTR markers in pigs.Mamm. Genome, 7: 433–437.
Signer, E. N.;Kuenzle, C. C.;Thomann, P. E.;Huebscher, U. 1988. Modified gel electrophoresis for higher resolution of DNA fingerprints.Nucl. Acids Res., 15:7739.
Stranzinger, G.;Dolf, G.;Fries, R.;Stocker, H. 1981. Some rare cases of chimerism in twin cattle and their proposed use in determining germinal cell migration.J. Heredity, 72: 360–362.
Tippett, P. 1983. Blood group chimaeras.Vox Sang, 44: 333–359.
Wislocki, G. B. 1939. Observations on twinning in marmosets.Amer. J. Anatomy, 64: 445–483.
Wong, Z.;Wilson, V.;Jeffreys, A. J.;Thein, S. L. 1986. Cloning a selected fragment from a human DNA “fingerprint”: isolation of an extremely polymorphic minisatellite.Nucl. Acids Res., 14: 4605–4616.
Wong, Z.;Wilson, V.;Patel, I.;Povey, S.;Jeffreys, A. J. 1987. Characterization of a panel of highly variable minisatellites cloned from human DNA.Ann. Human Genet., 51: 269–288.
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Signer, E.N., Anzenberger, G. & Jeffreys, A.J. Chimaeric and constitutive DNA fingerprints in the common marmoset (Callithrix jacchus). Primates 41, 49–61 (2000). https://doi.org/10.1007/BF02557461
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DOI: https://doi.org/10.1007/BF02557461