Skip to main content

Advertisement

SpringerLink
Log in

Cyclic nucleotides and the rapid inhibitions of bone45Ca uptake in response to bovine parathyroid hormone and 16,16-dimethyl prostaglandin E2 in chicks

  • Laboratory Investigations
  • Published:
Calcified Tissue International Aims and scope Submit manuscript

Summary

Intravenous injection of chicks with bovine parathyroid hormone (1–34) (3.3 μg/100 g body wt.) or 16,16-dimethyl PGE2 (5 μg/100 g body wt.) caused rapid (3 minute) net inhibition of45Ca uptake into femur and calvarium. These agents also elevated bone adenosine 3′,5′-cyclic monophosphate (cAMP) but not guanosine 3′,5′-cyclic monophosphate (cGMP) levels at this time. Methylxanthine phosphodiesterase inhibitors (MXPI), caffeine, theophylline, and 3-isobutyl-1-methylxanthine (IBMX) (0.3–5 mg/100 g body wt.) similarly inhibited net45Ca uptake into femur and to a lesser extent calvarium. Plasma45Ca and total Ca levels were unaltered or showed a slight tendency to be increased over control values 3 minutes after injection. However, the effects of the non-MXPI, dibutyryl-cAMP (0.5–5 mg/100 g body wt.) on bone45Ca uptake were negligible. Of the MXPI, only IBMX elevated total cAMP levels in chick bone at 3 minutes. These data implicate but do not confirm a mediatory role for cAMP in the rapid inhibitory actions of PTH and PGEs on bone net45Ca uptake in chicks.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. Shaw AJ, Dacke CG (1985) Evidence for a novel inhibition of calcium uptake into chick bone in response to bovine parathyroid hormone (1–34) or 16,16-dimethyl PGE2 in vivo. J Endocrinol 105:R5-R8

    PubMed  CAS  Google Scholar 

  2. Dacke CG, Shaw AJ (1987) Studies of the rapid effects of parathyroid hormone and prostaglandins on45Ca uptake into chick and rat bone in vivo. J Endocrinol 115:369–377

    PubMed  CAS  Google Scholar 

  3. Kenny AD, Dacke CG (1974) The hypercalcaemic response to parathyroid hormone in Japanese quail. Endocrinology 61:15–23

    Article  Google Scholar 

  4. Kirby GC, Dacke CG (1983) Hypercalcaemic responses to 16,16-dimethyl prostaglandin E2, a stable prostaglandin E2 analogue in chicks. J Endocrinol 99:115–122

    PubMed  CAS  Google Scholar 

  5. Shaw AJ, Dacke CG (1986) A comparison of the effects of bovine parathyroid hormone 1–34 and methylated prostaglandin E2 analogues on plasma calcium and inorganic phosphate levels in immature chickens and rats. Gen Comp Endocrinol 61:164–172

    Article  PubMed  CAS  Google Scholar 

  6. Peck WA, Klahr S (1979) Cyclic nucleotides in bone and mineral metabolism. In: Greengard P, Robison GA (eds) Advances in cyclic nucleotide research, vol 11. Raven Press, New York, pp 89–130

    Google Scholar 

  7. Walling MW, Marvaso V, Bernard GW (1978) Stimulation of guanylate cyclase activity in cultured osteogenic murine calvarial mesenchymal cells by PTH, calcitonin and insulin. Biochem Biophys Res Comm 83:521–527

    Article  PubMed  CAS  Google Scholar 

  8. Vaes G (1968) Parathyroid hormone-like action of N6-2′-O-dibutyryl adenosine-3′-5′ (cyclic)-monophosphate on bone explants in tissue culture. Nature 219:939–940

    Article  PubMed  CAS  Google Scholar 

  9. Wells H, Lloyd W (1969) Hypercalcaemic and hypophosphatemic effects of dibutyryl cyclic AMP in rats after parathyroidectomy. Endocrinol 83:861–867

    Google Scholar 

  10. Ivey JL, Wright DR, Tashjian AH (1976) Bone resorption in organ culture: inhibition by the divalent cation ionophores A23187 and X-537A. J Clin Invest 58:1327–1338

    Article  PubMed  CAS  Google Scholar 

  11. Tashjian AH, Ivey JL (1981) Stimulation of bone resorption in organ culture by cholera toxin. Biochem Biophys Res Comm 102:1055–1064

    Article  PubMed  CAS  Google Scholar 

  12. Lerner U, Gustafson GT (1979) Inhibitory effect of dibutyryl cyclic AMP on the release of calcium, inorganic phosphate and lysosomal enzymes from calvarial bones cultured for 24 hours. Acta Endocrinol 91:730–742

    PubMed  CAS  Google Scholar 

  13. Lerner U (1980), Inhibition of bone resorption and lysosomal enzyme release from calvarial bones cultured for 24 hours: synergism between cyclic AMP analogues and phosphodiesterase inhibitors. Acta Endocrinol 94:138–144

    PubMed  CAS  Google Scholar 

  14. Miyamoto E, Kuo JF, Greengard P (1969) Cyclic nucleotide-dependent protein kinases III. Purification and properties of adenosine 3′, 5′-monophosphate-dependent protein kinase from bovine brain. J Biol Chem 244:6395–6402

    PubMed  CAS  Google Scholar 

  15. Drummond GI, Powell CA (1970) Analogues of adenosine 3′, 5′-cyclic phosphate as activators of phosphorylase to kinase and as substrates for cyclic 3′,4′-nucleotide phosphodiesterase. Mol Pharmacol 6:24–29

    PubMed  CAS  Google Scholar 

  16. Heersche JNM, Fedak SA, Aurback GD (1971) The mode of action of dibutyryl adenosine 3′, 5′-monophosphate on bone tissue in vitro. J Biol Chem, 246:6770–6775

    PubMed  CAS  Google Scholar 

  17. Shaw AJ, Dacke CG (1987) Effect of phosphodiesterase inhibitors on45Ca uptake into chick bone in vivo: further evidence for a cAMP role in the rapid responses to parathyroid hormone and prostaglandins. J Endocrinol 112(suppl):100

    Google Scholar 

  18. Atkins D, Hunt NN, Ingleton PM, Martin TJ (1977) Rat osteogenic sarcoma cells: isolation and effects of hormones on the production of cyclic AMP and cyclic GMP. Endocrinol 101:555–561

    CAS  Google Scholar 

  19. Fletcher AE, Allan EH, Casley DJ, Martin TJ (1986) Atrial natriuretic factor receptors and stimulation of cyclic GMP formation in normal and malignant osteoblasts. FEBS Lett 208:263–268

    Article  PubMed  CAS  Google Scholar 

  20. Partridge NC, Kemp BE, Veroni MC, Martin TJ (1981) Activation of adenosine 3′, 5′-monophosphate-dependent protein kinase in normal and malignant bone cells by parathyroid hormone, prostaglandin E2 and prostacyclin. Endocrinol 108:220–225

    Article  CAS  Google Scholar 

  21. Liversey SA, Kemp BE, Re CA, Partridge NC, Martin TJ (1983), Selective hormonal activation of cyclic AMP-dependent protein kinase isoenzymes in normal and malignant osteoblasts. J Biol Chem 257:14983–14987

    Google Scholar 

  22. Rubin CS, Rangel-Aldao R, Sarkar D, Erlichman J, Fleischer N (1979) Characterisation and comparison of membrane-associated and cytosolic cAMP-dependent protein kinase. J Biol Chem 254:3797–3805

    PubMed  CAS  Google Scholar 

  23. Beavo JA, Rogers NL, Crofford OB, Hardman JG, Sutherland EW, Newman EV (1970) Effect of xanthine derivatives on lipolysis and on adenosine 3′, 5′-monophosphate phosphodiesterase activity. Mol Pharmacol 6:597–603

    PubMed  CAS  Google Scholar 

  24. Shaw AJ (1987) Studies on the effects of parathyroid hormone and prostaglandins on bone and calcium metabolism in chicks and immature rats in vivo. PhD Thesis, University of Aberdeen

  25. Marcus R, Orner FB (1980) Parathyroid hormone as a calcium ionophore in bone cells: tests of specificity. Calcif Tissue Int 32:207–211

    Article  PubMed  CAS  Google Scholar 

  26. Rasmussen H (1970) Cell communication, calcium ion and cyclic adenosine monophosphate. Science 170:404–412

    Article  PubMed  CAS  Google Scholar 

  27. Dunlop M, Larkins RG, Court JM (1981) Methylxanthine effects on cyclic adenosine 3′, 5′-monophosphate phosphodiesterase activity in preparations of neonatal rat cerebellum: modification by trifluoperazine. Biochem Biophys Res Comm 98:850–857

    Article  PubMed  CAS  Google Scholar 

  28. Cheun WY (1980) Calmodulin plays a pivotal role in cellular regulation. Science 207:19–27

    Article  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. School of Pharmacy and Biomedical Sciences, Portsmouth Polytechnic, St. Michael's Building, White Swan Rd., PO1 2DT, Portsmouth, UK

    A. J. Shaw & C. G. Dacke

Authors
  1. A. J. Shaw
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. C. G. Dacke
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Shaw, A.J., Dacke, C.G. Cyclic nucleotides and the rapid inhibitions of bone45Ca uptake in response to bovine parathyroid hormone and 16,16-dimethyl prostaglandin E2 in chicks. Calcif Tissue Int 44, 209–213 (1989). https://doi.org/10.1007/BF02556566

Download citation

  • Received:

  • Revised:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF02556566

Key words

Search

Navigation