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In vitro response of neonatal condylar cartilage to simultaneous exposure to the parathyroid hormone fragments 1–34, 28–48, and 53–84 hPTH

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Summary

Mandibular condylar explants of neonatal ICR mice were maintained as serum-free organ culture systems and were used to study the effects of three synthetic fragments of human parathyroid hormone (hPTH) on the morphology of the organ and its ability to incorporate [3H]thymidine. Forty-eight-hour incubation with hPTH (1–34), at a concentration of 0.5 μg/ml caused an increase of 88% in DNA synthesis and a marked increase in the size of the chondroprogenitor zone. The mitogenic effect of hPTH (1–34) was decreased to 34% over control levels when the fragment hPTH (28–48) was added to the system. However, the addition of the latter fragment brought about a marked enhancement in the mineralization of the cartilaginous extracellular matrix along with the formation of an appreciable amount of new bone. Thede novo osseous tissue was attached to the mineralized cartilage. When the carboxyl-terminal fragment hPTH (53–84) was added together with the other two fragments, the mitogenic effect of hPTH (1–34) was completely abolished and the respective cultures incorporated [3H]thymidine even less than untreated control cultures. Moreover, the addition of hPTH (53–84) to the culture system led to distinct structural features throughout the mineralized hypertrophic cartilage. The latter contained a mixture of cells within an unorganized extracellular matrix. Untreated control cultures lacked such structures, but contained the various cell zones as normally seen in neonatal condylar cartilage. Therefore, it seems reasonable to suggest that each of the three fragments tested induces a biological effect on neonatal cartilage and might be involved in the normal process of endochondral ossification.

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References

  1. Livne E, Silbermann M (1983) Changes in the structure and chemical composition of the mandibular condylar cartilage of the neonatal mouse. Archs Oral Biol 28:805–812

    Article  CAS  Google Scholar 

  2. Maor G, Hochberg Z, von der Mark K, Heinegard D, Silvermann M (1989) Human growth hormone enhances chondrogenesis and osteogenesis in a tissue culture system of chondroprogenitor cells. Endocrinology 125:1239–1245

    PubMed  CAS  Google Scholar 

  3. Maor G, Silbermann M (1981) In vitro effects of glucocorticoid hormones on synthesis of DNA in cartilage of neonatal mice. FEBS Letts 129:256–260

    Article  CAS  Google Scholar 

  4. Lewinson D, Silbermann M (1986) Parathyroid hormone stimulates proliferation of chondroprogenitor cells in vitro. Calcif Tissue Int 38:155–162

    Article  PubMed  CAS  Google Scholar 

  5. Silbermann M, Mirsky N, Levitan S, Weisman Y (1983) The effect of 1,25-dihydroxyvitamin D3 on cartilage growth in neonatal mice. Metab Bone Dis Rel Res 4:337–345

    CAS  Google Scholar 

  6. Maor G, Hochberg Z, Silbermann M (1989) Growth hormone and IGF-1 affect differently the growth and development of neonatal cartilage in vitro. 71th Ann Meet Endocr Soc, Abst #112

  7. Copray JCVM, Brouwer N, Prins APA, Jansen HWB (1988) Effects of polypeptide growth factors on mandibular condylar cartilage of the rat in vitro. J Biol Buccule 16:109–117

    CAS  Google Scholar 

  8. Maor G, Laron Z, Silbermann M (1989) In vitro effect of insulin-like growth factor I (IGF-1) and insulin on neonatal cartilage progenitor cells. Calcif Tissue Int 44:S11

    Article  Google Scholar 

  9. Livne E, Weiss A, Silbermann M (1989) Articular chondrocytes lose their proliferative activity with aging yet can be restimulated by PTH (1–84), PGE, and dexamethasone. J Bone Miner Res 4:529–548

    Google Scholar 

  10. Guinness-Hey M, Hock JM (1984) Increased trabecular bone mass in rats treated with human synthetic parathyroid hormone. Metab Bone Dis Rel Res 5:177–182

    Article  Google Scholar 

  11. Kawashima K, Iwata S, Endo H (1980) Growth stimulative effect of parathyroid hormone, calcitonin and N6, O2′-dibutyrl adenosine 3′,5′-cyclic monophosphoric acid on chick embryonic cartilage cultivated in a chemically defined medium. Endocrinol Japon 27:349–356

    CAS  Google Scholar 

  12. Burch WM, Lebovitz HE (1983) Parathyroid hormone stimulates growth of embryonic chick pelvic cartilage in vitro. Calcif Tissue Int 35:526–532

    Article  PubMed  CAS  Google Scholar 

  13. Shimshoni Z, Binderman I, Fine N, Somjen D (1984) Mechanical and hormonal stimulation of cell cultures derived from young rat mandible condyle. Archs Oral Biol 29:827–831.

    Article  CAS  Google Scholar 

  14. Levy J, Shimshoni Z, Somjen D, Berger E, Fine N, Silbermann M, Binderman I (1988) Rat epiphyseal cells in culture: responsiveness to bone-seeking hormones. In Vitro Cell Dev Biol 24:620–624

    PubMed  CAS  Google Scholar 

  15. Kato Y, Koike T, Iwamoto M, Kinoshita M, Sato K, Hiraki Y, Suzuki F (1988) Effects of limited exposure of rabbit chondrocytes cultures to parathyroid hormone and dibutyryl adenosine 3′,5′-monophosphate on cartilage characteristic proteoglycan synthesis. Endocrinology 122:1991–1997

    PubMed  CAS  Google Scholar 

  16. Pines M, Hurwitz S (1988) The effect of parathyroid hormone and atrial natriuretic peptide on cyclic nucleotides production and proliferation of avian epiphyseal growth plate chondroprogenitor cells. Endocrinology 123:360–365

    PubMed  CAS  Google Scholar 

  17. Canterbury JM, Reiss E (1972) Multiple immunoreactive molecular forms of parathyroid hormone in human plasma. Proc Soc Exp Biol Med 140:1393–1398

    PubMed  CAS  Google Scholar 

  18. Habener JF, Serge GV, Powell D, Murray TM, Potts JT (1972) Immunoreactive parathyroid hormone in circulation of man. Nat New Biol 238:152–154

    Article  PubMed  CAS  Google Scholar 

  19. Arnaud CD, Goldsmith RS, Bordier PJ, Sizemore GW (1974) Influence of immunoheterogeneity of circulating parathyroid hormone on results of radioimmunoassays of sera in man. Am J Med 56:785–793

    Article  PubMed  CAS  Google Scholar 

  20. Martin KJ, Freitag JJ, Conrades MB, Hruska KA, Klahr S, Slatopolsky E (1978) Selective uptake of the synthetic amino terminal fragment of bovine parathyroid hormone by isolated perfused bone. J Clin Invest 62:256–261

    Article  PubMed  CAS  Google Scholar 

  21. Parsons JA, Robinson CJ (1968) A rapid indirect hypercalcemic action of parathyroid hormone demonstrated in isolated perfused bone. In: Talmage RV, Belanger LF (eds) Parathyroid hormone and thyrocalcitonin (Calcitonin). Excerpta Medica, Amsterdam, pp 329–331

    Google Scholar 

  22. Gaillard PJ, Wassenaar AM, Van Wijhe-Wheeler ME (1977) Effects of parathyroid hormone and a synthetic fragment (PTH 1–34) on bone in vitro. Proc Konin Nederl Akad 80:267–280

    CAS  Google Scholar 

  23. Takigawa M, Okada M, Takano T, Ohmae H, Sakuda M, Suzuki F (1984) Studies on chondrocytes from mandibular condylar cartilage, nasal septal cartilage, and spenooccipital synchondrosis in culture. I. Morphology, growth, glycosaminoglycan synthesis, and responsiveness to bovine parathyroid hormone (1–34). J Dent Res 63:19–22

    PubMed  CAS  Google Scholar 

  24. Shurtz-Swirski R, Lewinson D, Shenzer P, Mayer H, Silbermann M (1990) Effects of parathyroid hormone fragments on the growth of murine cartilage in vitro. Acta Endocrinol 122:217–226

    PubMed  CAS  Google Scholar 

  25. Lewinson D (1989) Ferrocyanide-reduced osmium method for mineralizing cartilage: further evidence for the enhancement of intracellular glycogen and visualization of matrix components. Histochem J 21:259–270

    Article  PubMed  CAS  Google Scholar 

  26. Silbermann M, Tennenbaum H, Livne E, Leapman R, von der Mark, Reddi AH (1987) In vitro behaviour of fetal condylar cartilage in serum-free hormone supplemented medium. Bone 8:117–126

    Article  PubMed  CAS  Google Scholar 

  27. Chin JE, Schalk EM, Kemick MS, Wuthier RE (1986) Effect of synthetic human parathyroid hormone on the level of alkaline phosphatase activity and formation of alkaline phosphatase-rich matrix vesicles by primary cultures of chicken epiphyseal growth plate chondrocytes. Bone Miner 1:421–427

    PubMed  CAS  Google Scholar 

  28. Silve CM, Hradek GT, Jones AL, Arnaud CD (1982) Parathyroid hormone receptors in intact embryonic chicken bone: characterization and cellular localization. J Cell Biol 94:379–383

    Article  PubMed  CAS  Google Scholar 

  29. Murray TM, Rao LG, Muzaffar SA, Ly H (1989) Human parathyroid hormone carboxyterminal peptide (53–84) stimulates alkaline phosphatase activity in dexamethasone-treated rat osteosarcoma cells in vitro. Endocrinology 124:1097–1099

    PubMed  CAS  Google Scholar 

  30. Schluter KD, Hellstern H, Wingender E, Mayer H (1989) The central part of parathyroid hormone stimulates thymidine incorporation of chondrocytes. J Biol Chem 264:11087–11092

    PubMed  CAS  Google Scholar 

  31. Wingender E, Bercz G, Blocker H, Frank R, Mayer H (1989) Expression of human parathyroid hormone in escherichia coli. J Biol Chem 264:4367–4373

    PubMed  CAS  Google Scholar 

  32. Kaye AM, Weisman Y, Harell A, Somjen D (in press) Hormonal stimulation of bone cell proliferation. J Steroid Biochem

  33. Wong GL (1986) Skeletal effects of parathyroid hormone. In: Peck WA (ed) Bone and mineral research, vol. 4. Elsevier, Amsterdam, pp 103–129

    Google Scholar 

  34. van Leeuwen JPTM, Bos MP, Lowik CWGM, Herrmann-Erlee MPM (1988) Effect of parathyroid hormone and parathyroid hormone fragments on the intracellular ionized calcium concentration in an osteoblast cell line. Bone Miner 4:177–188

    PubMed  Google Scholar 

  35. Sugimoto T, Fukase M, Tsutsumi M, Imai Y, Hishikawa Y, Yoshimoto Y, Fujita T (1985) Additive effects of parathyroid hormone and calcitonin on adenosine 3′, 5′ monophosphate release in newly established perfusion system of rat femur. Endocrinology 117:1901–1905.

    PubMed  CAS  Google Scholar 

  36. Galceran T, Slatopolsky E, Martin KJ (1987) Differences in the response to intact b-PTH 1-84 and synthetic b-PTH 1-34 in isolated perfused bones from young and adult dogs. Calcif Tissue Int 41:290–292

    Article  PubMed  CAS  Google Scholar 

  37. Zanelli JM, Lane E, Kimura T, Sakakibara S (1985) Biological activities of synthetic human parathyroid hormone (PTH) 1–84 relative to natural 1-84 PTH in two different in vivo bioassay systems. Endocrinology 117:1962–1967

    PubMed  CAS  Google Scholar 

  38. Habener JF, Potts JT Jr (1976) Chemistry, biosynthesis, secretion and metabolism of parathyroid hormone. In: Aurbach GD (ed) Handbook of physiology, vol. 7. Am Physiol Soc, Williams and Wilkins, Baltimore, pp 313–342

    Google Scholar 

  39. McKee MD, Murray TM (1985) Binding of intact parathyroid hormone to chicken renal plasma membranes: evidence for a second binding site with carboxyl terminal specificity. Endocrinology 117:1930–1939

    PubMed  CAS  Google Scholar 

  40. Rao LG, Murray TM (1985) Binding of intact parathyroid hormone (1–84) to rat osteosarcoma cells: major contribution of binding sites for the carboxyl-terminal region of the hormone. Endocrinology 117:1632–1638.

    PubMed  CAS  Google Scholar 

  41. Tam CS, Heersche JNM, Murray TM, Parsons JA (1982) Parathyroid hormone stimulates the bone apposition rate independently of its resorptive action: differential effects of intermittent and continuous administration. Endocrinology 110:506–512

    Article  PubMed  CAS  Google Scholar 

  42. Slovik DM, Rosenthal DI, Doppelt SH, Potts JT Jr, Daly MA, Campbell JA, Neer RM (1986) Restoration of spinal bone in osteoporotic men by treatment with human parathyroid hormone (1–34) and 1,25 dihydroxy-vitamin D. J Bone Miner Res 1:377–381

    PubMed  CAS  Google Scholar 

  43. Hock JM, Hummert JR, Boyce R, Fonseca J, Raisz LG (1989) Resorption is not essential for the stimulation of bone growth by hPTH (1–34) in rats in vivo. J Bone Miner Res 4:449–458

    Article  PubMed  CAS  Google Scholar 

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Authors and Affiliations

  1. Laboratory for Musculoskeletal Research, The Rappaport Family Institute for Research in the Medical Sciences, Faculty of Medicine, Technion-Israel Institute of Technology, P.O. Box 9649, Haifa, Israel

    M. Silbermann, R. Shurtz-Swirski, D. Lewinson & P. Shenzer

  2. The Department of Genetics, GBF, Braunschweig, Federal Republic of Germany

    H. Mayer

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  1. M. Silbermann
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  2. R. Shurtz-Swirski
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  3. D. Lewinson
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  4. P. Shenzer
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  5. H. Mayer
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Silbermann, M., Shurtz-Swirski, R., Lewinson, D. et al. In vitro response of neonatal condylar cartilage to simultaneous exposure to the parathyroid hormone fragments 1–34, 28–48, and 53–84 hPTH. Calcif Tissue Int 48, 260–266 (1991). https://doi.org/10.1007/BF02556377

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  • DOI: https://doi.org/10.1007/BF02556377

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