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Osteogenesis by canine and rabbit bone marrow in diffusion chambers

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Summary

Osteogenic activity of canine and rabbit bone marrow and marrow stromal fibroblasts (MSF) derived from marrow culturedin vitro was evaluated using diffusion chambers. Marrow from young dogs and rabbits grown in cell culture produced confluent layers of MSF. Diffusion chambers containing 0.18–7.6×106 allogeneic MSF were inserted into the peritoneal cavities of 5 dogs and 6 rabbits. Chambers recovered from the dogs (15/16) contained only loose fibrous tissue while chambers from rabbits (9/13) contained membranous bone and cartilage. Diffusion chambers implanted with 1.0–32.4×107 fresh allogeneic marrow cells suspended in cell culture medium were inserted into the peritoneal cavities of 11 dogs and 9 rabbits for 3–8 weeks, and after recovery examined histologically. Membranous bone was formed in 4/40 chambers containing canine marrow while bone and hyaline cartilage was formed in 21/27 chambers containing rabbit marrow. This apparent species difference in incidence of bone marrow osteogenesis may relate to a lower concentration of osteogenic precursor cells in canine marrow, a failure of osteogenic precursor cells to differentiate to osteoblasts in a somewhat artificial environmentin vivo (viz diffusion chambers), a lack of cell-matrix interaction to stimulate cell differentiation, inappropriately short diffusion chamber implantation times post grafting, or a difference in ontogenetic stage of development of marrow donors with rabbit cells being physiologically younger.

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Author notes

  1. Kenneth A. Johnson

    Present address: Department of Clinical Sciences, College of Veterinary Medicine and Biomedical Sciences Colorado State University, 80523, Fort Collins, CO

Authors and Affiliations

  1. Department of Veterinary Clinical Studies, The University of Sydney, 2006, Sydney, Australia

    Kenneth A. Johnson & Christopher R. Bellenger

  2. School of Pathology, University of New South Wales, 2033, Sydney, Australia

    C. Rolfe Howlett

  3. Department of Zoology, The University of Sydney, 2006, Sydney, Australia

    Patricia Armati-Gulson

Authors
  1. Kenneth A. Johnson
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  2. C. Rolfe Howlett
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  3. Christopher R. Bellenger
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  4. Patricia Armati-Gulson
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Johnson, K.A., Howlett, C.R., Bellenger, C.R. et al. Osteogenesis by canine and rabbit bone marrow in diffusion chambers. Calcif Tissue Int 42, 113–118 (1988). https://doi.org/10.1007/BF02556343

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  • DOI: https://doi.org/10.1007/BF02556343

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