Summary
L-lysine, a cationic essential amino acid, has been reported to affect calcium transport in both intestine and kidney. In order to investigate whether this effect is associated with changes in cytosolic calcium homeostasis, we studied the effect of L-lysine deprivation on intracellular calcium concentration ([Ca2+]i), as well as45Ca efflux and accumulation in normal human fibroblasts. Steady state [Ca2+]i, measured using fura-2 fluorescence in cells cultured for 18 hours in a L-lysine-free medium, was significantly higher than in cells grown in the presence of as little as 4 μM L-lysine. L-lysine deprivation also led to a significant decrease of45Ca fractional efflux compared with cells grown in complete medium. This effect was paralleled by a significant decrease in45Ca accumulation. Lack of L-arginine from the growth medium for the same time period had no effect on either [Ca2+]i,45Ca efflux, or45Ca accumulation rate. Presumably, the lack of L-lysine for a significant amount of time impairs the active mechanisms of calcium extrusion, which is only partially compensated by a reduction of calcium accumulation rate. This leads to an increased steady state [Ca2+]i. It is concluded that L-lysine is an important modulator of cytosolic calcium homeostasis.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Recker RR, Saville PD, Heaney RP (1977) Effect of estrogens and calcium carbonate on bone loss in postmenopausal women. Ann Int Med 87:649–655
Riis B, Thomsen K, Christiansen C (1987) Does calcium supplementation prevent postmenopausal bone loss? N Engl J J Med 316:173–177
Caniggia A, Gennari C, Bianchi V, Guideri R (1963) Intestinal absorption of45Ca in senile osteoporosis. Acta Med Scand 173:613–617
Gallagher JC, Riggs BL, Eisman J, Hamstra A, Arnaud SB, DeLuca HF (1979) Intestinal calcium absorption and serum vitamin D metabolites in normal subjects and osteoporotic patients. Effect of age and dietary calcium. J Clin Invest 64:729–736
Avioli LV (1987) The calcium controversy and the recommended dietary allowance. In: Avioli LV (ed) The osteoporotic syndrome. Detection, prevention and treatment, 2nd ed. Grune and Stratton, Orlando, FL, pp 57–66
Wilkinson R (1976) Absorption of calcium, phosphorus and magnesium. In: Nordin BEC (ed) Calcium, phosphate and magnesium metabolism. Clinical physiology and diagnostic procedures, Churchill Livingstone, Edinburgh, UK, pp 36–112
McBean LD, Speckman EW (1974) A recognition of the interrelationship of calcium with various dietary components. Am J Clin Nutr 27:603–609
Wasserman RH, Comar CL, Nold MM (1956) The influence of amino acids and other organic compounds on the gastro-intestinal absorption of calcium45 and strontium89 in the rat. J Nutr 59:371–383
Wasserman RH, Comar CL, Schooley JC, Lengemann FW (1957) Interrelated effects of L-lysine and other dietary factors on the gastrointestinal absorption of calcium45 in the rat and chick. J Nutr 62:367–376
Raven AM, Lengemann FW, Wasserman RH (1960) Studies of the effect of lysine on the absorption of radiocalcium and radiostrontium by the rat. J Nutr 72:29–36
Tardivel S, Toure A, Digaud A, Fournier P (1980) Relations entre la phosphorylation de molecules aminees et leur activite sur l'absorption du calcium. Reprod Nutr Develop 20:1379–1385
Landiharintsoa L (1980) Phosphorylation d'acides D- et L-amines par la muqueuse ileale en relation avec l'absorption du calcium. J Physiol, Paris 76:899–901
Dupuis Y, Tardivel S, Digaud A, Fournier P (1980) The influence of phosphorylable molecules on calcium transfer in the rat ileum. Life Sciences 26:899–907
Wolinsky I, Fosmire GJ (1982) Calcium metabolism in aged mice ingesting a lysine-deficient diet. Gerontology 28:156–162
Grynkiewicz G, Poenie M, Tsien RY (1985) A new generation of Ca2+ indicators with greatly improved fluorescence properties. J Biol Chem 260:3440–3450
Borle AB, Uchikawa T, Anderson JH (1982) Computer simulation and interpretation of45Ca efflux profile patterns. J Membr Biol 68:37–46
Takuwa N, Takuwa Y, Bollag WE, Rasmussen H (1987) The effect of bombesin on polyphosphoinositide and calcium metabolism in Swiss 3T3 cells. J Biol Chem 262:182–188
Ikemoto N (1982) Structure and function of the calcium pump protein of sarcoplasmic reticulum. Ann Rev Physiol 44:297–317
Maruyama K, McLennan DH (1988) Mutation of aspartic acid-351, lysine-352 and lysine-515 alters the Ca2+ transport activity of the Ca2+-ATPase expressed in COS-1 cells. Proc Natl Acad Sci USA 85:3314–3318
Dupuis Y, Crouzoulon G, Landiharintsoa L, Toure A, Fournier P (1984) Enterocyte microvillus can phosphorylate molecules which inhibit endogenous phosphorylation of its proteins. Arch Intl de Physiologie et de Biochimie 92:1–11
Dupuis Y, Crouzoulon G, Fournier P (1981) Does the inhibition of microvillus protein phosphorylation by lysine explain the activity of the latter on calcium transfer? Int J Biochem 13:1163–1170
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Civitelli, R., Fedde, K.N., Harter, J. et al. Effect of L-lysine on cytosolic calcium homeostasis in cultured human normal fibroblasts. Calcif Tissue Int 45, 193–197 (1989). https://doi.org/10.1007/BF02556063
Received:
Revised:
Issue Date:
DOI: https://doi.org/10.1007/BF02556063