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Prolonged and ubiquitous inhibition by interferon γ of bone resorption induced by parathyroid hormone-related protein, 1,25-dihydroxyvitamin D3, and interleukin 1 in fetal mouse forearm bones

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Summary

To investigate the mechanism of the inhibitory effects of interferon-γ (IFN-γ) on bone resorption, the effects of murine IFN-γ on45Ca release from prelabeled fetal mouse forearm bones were investigated. Murine IFN-γ usually did not affect basal45Ca release but almost completely and equipotently inhibited bone resorption induced by PTH(1-34), PTH-rP(1-34), 1,25(OH)2D3, and interleukin 1 (IL-1). The half-maximal concentration for inhibition of bone resorption induced by IL-1α was 25.8±14.6 U/ml (mean±SD for 13 experiments), which is not different from those for PTH, PTH-rP, and 1,25(OH)2D3. There was no correlation between prostaglandin E2 concentration in the conditioned medium and45Ca release from the IFN-γ-treated forearm bones. The inhibitory effect of IFN-γ on bone resorption induced by PTH-rP (1-34) or IL-1α continued during 6 days of culture, whereas that of calcitonin disappeared after 2 days of culture. These findings suggest that IFN-γ nonpreferentially inhibits bone resorption induced by various bone-resorbing factors in fetal mouse forearm bones via a PGE2-independent mechanism. As no escape phenomenon developed in IFN-γ-treated bones, the cytokine may be potentially useful for treatment of certain patients with malignancy-associated hypercalcemia.

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Author notes

  1. Toru Fujii

    Present address: The Third Department of Internal Medicine, Nagoya City University Medical School, Nagoya, Japan

Authors and Affiliations

  1. Institute of the Foundation for Growth Science in Japan, Wakamatsu-cho, Shinjuku-ku, 162, Tokyo, Japan

    Yuko Fujii & Tomoko Satoh

  2. Institute of Clinical Endocrinology, Tokyo Women's Medical College, Kawada-cho 8-1, Shinjuku-ku, Tokyo, Japan

    Kanji Sato, Keizo Kasono, Toru Fujii & Kazuo Shizume

Authors
  1. Yuko Fujii
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  2. Kanji Sato
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  3. Keizo Kasono
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  4. Tomoko Satoh
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  5. Toru Fujii
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  6. Kazuo Shizume
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Fujii, Y., Sato, K., Kasono, K. et al. Prolonged and ubiquitous inhibition by interferon γ of bone resorption induced by parathyroid hormone-related protein, 1,25-dihydroxyvitamin D3, and interleukin 1 in fetal mouse forearm bones. Calcif Tissue Int 47, 178–182 (1990). https://doi.org/10.1007/BF02555985

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  • DOI: https://doi.org/10.1007/BF02555985

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