Summary
Previous work has shown that vitamin D3 or 1,25-dihydroxy-vitamin D3 affect calcium content and fluxes in mitochondria of chick skeletal musclein situ. Studies were performed to investigate whether these effects are related to variations in the Ca2+ transport properties of mitochondrial membranes. Mitochondria isolated from skeletal muscle of vitamin D-deficient chicks and chicks dosed with 1,25(OH)2D3 for 3 or 7 days (50 ng/day) were employed. No changes in the rate and affinity for calcium of the Ruthenium Red-sensitive Ca2+ uptake system were detected after treatment with 1,25(OH)2D3. The metabolite did not cause either modifications in Ca2+ efflux from mitochondria preloaded with the cation induced by Na+ or blockage of mitochondria energy supply. Prior treatment of animals with vitamin D3 was also without effects. However, a significant stimulation of Ca2+ uptake by intact muscle preparations from the same experimental animals was observed in response to treatment with 1,25(OH)2D3 in vivo (50 ng/day, 3 days) orin vitro (10−10 M, 60 minutes). In addition, the Ca content of muscle mitochondria was markedly diminished in chicks treated with the sterol. It is suggested that the effects of 1,25(OH)2D3 on muscle mitochondrial Ca metabolism may be secondary to changes in cytoplasmic Ca2+.
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References
Schott GD, Wills MR (1976) Muscle weakness in osteomalacia. Lancet i:626–629
Floyd M, Ayyard DR, Barwick DD, Hudgson P, Weightman D (1974) Myopathy in chronic renal failure. Q J Med 43:509–523
Henderson RG, Russell RG, Ledingham JGG, Smith R, Oliver DO, Walton RJ, Small DG, Preston C (1974) Effects of 1,25-dihydroxycholecalciferol on calcium absorption in renal failure. Lancet i:379–384
Boland R (1986) Role of vitamin D in skeletal muscle function. Endocrine Rev 7:434–447
Peacock M, Heyburn PJ (1977) Effect of vitamin D3 metabolites on proximal muscle weakness. Calcif Tissue Res 24(suppl):R20
Weber A, Reiss I (1964) Role of calcium on contraction and relaxation of muscle. Fed Proc 23:896–900
Matthews C, Heimberg KW, Ritz E, Agostini B, Fritzche J, Hasselbach W (1977) Effect of 1,25-dihydroxycholecalciferol on impaired calcium transport by sarcoplasmic reticulum in experimental uremia. Kidney Int 11:227–235
Boland AR, Gallego S, Boland R (1983) Effects of vitamin D3 on phosphate and calcium transport across and composition of skeletal muscle plasma cell membranes. Biochim Biophys Acta 733:264–273
Smith R, Stern G (1967) Myopathy, osteomalacia and hyperparathyroidism. Brain 90:593–602
Giuliani D, Boland RL (1984) Effects of vitamin D3 metabolites on calcium fluxes in intact chicken skeletal muscle and myoblast culturedin vitro. Calcif Tissue Int 36:200–205
Boland AR, Boland R (1985)In vitro cellular muscle calcium metabolism. Characterization of effects of 1,25-dihydroxy-vitamin D3 and 25-hydroxy-vitamin D3. Z Naturforsch 40c:102–108
Pleasure D, Wysynski A, Summer D, Schotland B, Feldmann B, Nugent N (1979) Skeletal muscle calcium metabolism and contractile force in vitamin D-deficient chicks. J Clin Invest 64:1157–1167
Noack EA, Hemen EM (1977) A kinetic study of calcium transport by heart mitochondria. Eur J Biochem 79:245–250
Hayat LH, Crompton M (1982) Evidence for the existence of regulatory sites for Ca2+ on the Na+/Ca2+ carrier of cardiac mitochondria. Biochem J 202:509–518
Crompton M, Moser R, Lüdi H, Carafoli E (1978) The interrelations between the transport of sodium and calcium in mitochondrial of various mammalian tissues. Eur J Biochem 82:25–31
Wasserman RH, Taylor AM (1973) Intestinal absorption of phosphate in chick: effect of vitamin D3 and other parameters. J Nutr 103:586–599
Norman A, Roberts P (1980) Steroid competition assay for determination of 25-hydroxyvitamin D and 24,25-dihydroxyvitamin D. Methods Enzymol 67:473–478
Makinen MW, Lee CP (1968) Biochemical studies of skeletal muscle mitochondria. 1. Microanalysis of cytochrome content, oxidative and phosphorylative activities of mammalian skeletal muscle mitochondria. Arch Biochem Biophys 126:75–82
Chappell JB, Perry SV (1954) Biochemical and osmotic properties of skeletal muscle mitochondria. Nature 173:1094–1096
Boland AR, Albornoz LE, Boland R (1983) The effect of cholecalciferolin vivo on protein and lipids of skeletal muscle from rachitic chicks. Calcif Tissue Int 35:798–805
Reed K, Bygrave FL (1974) The inhibition of mitochondrial calcium transport by lanthanides and Ruthenium Red. Biochem J 140:143–155
Lowry OH, Rosembrough NJ, Farr AL, Randall RJ (1951) Protein measurement with Folin phenol reagent. J Biol Chem 193:265–275
Ash GR, Bygrave FL (1977) Ruthenium Red as a probe in assessing the potential of mitochondria to control intracellular calcium in liver. FEBS Lett 78:166–168
Bikle DD, Askew EW, Zolock DT, Morrisey RL, Herman RH (1980) Calcium accumulation by chick intestinal mitochodria. Regulation by vitamin D3 and 1,25-dihydroxyvitamin D3. Biochim Biophys Acta 598:561–574
Boland AR, Boland R (1987) Rapid changes in skeletal muscle calcium uptake inducedin vitro by 1,25-dihydroxyvitamin D3, are suppressed by clacium channel blockers. Endocrinology 120:1858–1864
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Selles, J., Boland, R.L. In vitro calcium transport properties of skeletal muscle mitochondria from vitamin D-deficient and 1,25-dihydroxy-vitamin D3-treated chicks. Calcif Tissue Int 47, 46–50 (1990). https://doi.org/10.1007/BF02555865
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DOI: https://doi.org/10.1007/BF02555865