Calcified Tissue International

, Volume 49, Issue 6, pp 421–426 | Cite as

Expression of bone sialoprotein (BSP) in developing human tissues

  • Paolo Bianco
  • Larry W. Fisher
  • Marian F. Young
  • John D. Termine
  • Pamela Gehron Robey
Molecular and Cellular Biology

Summary

Bone sialoprotein (BSP) and its messenger RNA were localized in developing human skeletal and nonskeletal tissues by means of immunohistochemistry andin situ hybridization. Both protein and mRNA were found in mature, bone-forming cells but not in their immature precursors. In addition, osteoclasts displayed positive immunostaining and high densities of autoradiographic grains byin situ hybridization experiments. BSP was expressed in fetal epiphyseal cartilage cells, particularly in hypertrophic chondrocytes of growth plates. Though neither the protein nor the mRNA were identified in a variety of other connective and nonconnective tissues, an unexpected finding was the expression of BSP in the trophoblast cells of placenta. These findings show that BSP is primarily an osteoblast-derived component of the bone matrix expressed at late stages of differentiation. We have also found that osteoclasts produce BSP, possibly as a mediator of cell attachment to bone.

Key words

Bone sialoprotein Developing bone Noncollagenous proteins In situ hybridization Osteoblast Osteoclast Cell adhesion 

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. 1.
    Herring GM (1972) The organic matrix of bone. In: Bourne GH (ed) The biochemistry and physiology of bone, vol 1. Academic Press, New York, pp 127–189Google Scholar
  2. 2.
    Fisher LW, Whitson SW, Avioli LV, Termine JD (1983) Matrix sialoprotein of developing bone. J Biol Chem 258:12723–12727PubMedGoogle Scholar
  3. 3.
    Fisher LW, Termine JD, Young MF (in press) Human bone sialoprotein: deduced protein sequence and chromosomal localization. J Biol ChemGoogle Scholar
  4. 4.
    Franzen A, Heinegard D (1985) Isolation and characterization of two sialoproteins present only in bone calcified matrix. Biochem J 232:715–724PubMedGoogle Scholar
  5. 5.
    Fisher L, Hawkins GR, Tuross N, Termine JD (1987) Purification and partial characterization of bone proteoglycans I and II, bone sialoproteins I and II and osteonectin from the mineral compartment of human bone. J Biol Chem 262:9702–9708PubMedGoogle Scholar
  6. 6.
    Kinne RW, Fisher LW (1987) Keratan sulfate proteoglycan in rabbit compact bone is bone sialoprotein II. J Biol Chem 262:10206–10211PubMedGoogle Scholar
  7. 7.
    Oldberg A, Franzen A, Heinegard D (1988) The primary structure of a cell-binding bone sialoprotein. J Biol Chem 263:19430–19432PubMedGoogle Scholar
  8. 8.
    Ruoslahti E, Pierschbacher MD (1987) New perspectives in cell adhesion: RGD and integrins. Science 238:491–497PubMedCrossRefGoogle Scholar
  9. 9.
    Oldberg A, Franzen A, Heinegard D, Pierschbacher M, Ruoslahti E (1988) Identification of a bone sialoprotein receptor in osteosarcoma cells. J Biol Chem 263:19433–19436PubMedGoogle Scholar
  10. 10.
    Somerman MJ, Fisher LW, Foster RA, Sauk JJ (1988) Human bone sialoproteins I and II enhance fibroblast attachment in vitro. Calcif Tissue Int 43:50–53PubMedCrossRefGoogle Scholar
  11. 11.
    Mintz KP, Midura RJ, Gehron Robey P, Termine JD, Fisher LW (1990) Attachment properties of non-denatured rat bone sialoprotein. J Bone Miner Res 5:S232Google Scholar
  12. 12.
    Ecarot-Charrier B, Bouchard S, Delloye C (1989) Bone sialoprotein II synthesized by cultured osteoblasts contains tyrosine sulfate. J Biol Chem 264:20049–20053PubMedGoogle Scholar
  13. 13.
    Midura RJ, McQuillan DJ, Benham KJ, Fisher LW, Hascall VC (1990) A rat osteogenic cell line (UMR 106-01) synthesizes a highly sulfated form of bone sialoprotein. J Biol Chem 265:5285–5291PubMedGoogle Scholar
  14. 14.
    Nomura S, Wills AJ, Edwards DR, Heath JK, Hoga BLM (1988) Developmental expression of 2ar (osteopontin) and SPARC (osteonectin) RNA as revealed by in situ hybridization. J Cell Biol 106:441–450PubMedCrossRefGoogle Scholar
  15. 15.
    Graham RC, Karnovsky MJ (1966) The early stages of absorption of horseradish peroxidase in the proximal tubules of mouse kidneys: ultrastructural cytochemistry by a new technique. J Histochem Cytochem 14:291–390PubMedGoogle Scholar
  16. 16.
    Cox KH, DeLeon DV, Angerer LM, Angerer RC (1984) Detection of mRNAs in sea urchin embryos by in situ hybridization using asymmetric RNA probes. Dev Biol 101:485–502PubMedCrossRefGoogle Scholar
  17. 17.
    Mark MP, Prince CW, Oosawa T, Gay S, Bronckers ALJJ, Butler WT (1987) Immunohistochemical demonstration of a 44-kD phosphoprotein in developing rat bones. J Histochem Cytochem 35:707–715PubMedGoogle Scholar
  18. 18.
    Mark MP, Butler WT, Prince CW, Finkelman RD, Ruch JV (1988) Developmental expression of 44-kD bone phosphoprotein (osteopontin) and bone-carboxyglutamic acid (Gla) containing protein (osteocalcin) in calcifying tissues of rat. Differentiation 37:123–136PubMedCrossRefGoogle Scholar
  19. 19.
    Bianco P, Silverstrini G, Termine JD, Bonucci E (1988) Immunohistochemical localization of osteonectin in human and calf developing bone using monoclonal antibodies. Calcif Tissue Int 43:155–161PubMedGoogle Scholar
  20. 20.
    Bianco P, Fisher LW, Young MF, Kopp JB, Termine JD, Gehron Robey P (1990) The use of synthetic peptide antibodies and in situ hybridization for investigating expression and localization of small proteoglycans of developing bone (biglycan and decorin). In: Cohn DV, Glorieux FH, Martin TJ (eds) Proceedings ASBMR/ICCRH. Elsevier, Amsterdam, pp 201–206Google Scholar
  21. 21.
    Baron R, Neff L, Louvard D, Courtoy JP (1985) Cell-mediated extracellular acidification and bone resorption: evidence for a low pH in resorbing lacunae and localization of a 100-kD lysosomal membrane protein at the osteoclast ruffled border. J Cell Biol 101:2210–2222PubMedCrossRefGoogle Scholar
  22. 22.
    Metsaranta M, Young MF, Sandberg M, Termine JD, Vuorio E (1989) Localization of osteonectin expression in human fetal skeletal tissues by in situ hybridization. Calcif Tissue Int 45:146–152PubMedCrossRefGoogle Scholar

Copyright information

© Springer-Verlag 1991

Authors and Affiliations

  • Paolo Bianco
    • 1
  • Larry W. Fisher
    • 1
  • Marian F. Young
    • 1
  • John D. Termine
    • 1
  • Pamela Gehron Robey
    • 1
  1. 1.Bone Research Branch, National Institute of Dental ResearchNational Institutes of HealthBethesdaUSA

Personalised recommendations