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Inhibition of calcium pyrophosphate dihydrate crystal formation: Effects of carboxylate ions

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Summary

Proteoglycans are recognized to inhibit calcium pyrophosphate dihydrate (CPPD) crystal formation but the mechanisms are not known. To study the role of carboxylate (−CO2 −) ligands, the possible inhibitor effects of sodium acetate, sodium D-glucuronate, disodium malate, and trisodium citrate were studied using solution mixtures containing [Ca2+]=1.5 mM, [Mg2+]=0.5 mM, [PPi] =0.1 mM, [Pi]=0.1 mM, [Na+]=140 mM, 37°C, pH 7.4 with or without 9.5±0.5 mg CPPD (seed) crystals. These studies showed that monocarboxylates (acetate, glucuronate) have little inhibitive effect. Progressively greater inhibition was found with dicarboxylate (malate) and tricarboxylate (citrate) indicating that the arrangement of carboxylate (and sulfate) ligands on proteoglycan is more important than the inhibitory effect of individual ligands.

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Authors and Affiliations

  1. Department of Pathology, Mount Sinai Hospital, 600 University Avenue, M5G 1X5, Toronto, Ontario, Canada

    Pei-Tak Cheng Ph.D. & Kenneth P. H. Pritzker

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  1. Pei-Tak Cheng Ph.D.
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  2. Kenneth P. H. Pritzker
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Cheng, PT., Pritzker, K.P.H. Inhibition of calcium pyrophosphate dihydrate crystal formation: Effects of carboxylate ions. Calcif Tissue Int 42, 46–52 (1988). https://doi.org/10.1007/BF02555838

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  • DOI: https://doi.org/10.1007/BF02555838

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