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Bone formation by osteoblast-like cells in a three-dimensional cell culture

  • Molecular and Cellular Biology
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Summary

Cells of the clonal osteogenic cell line MC3T3-E1 were seeded onto a three-dimensional matrix of denatured collagen type 1 and cultured for a period of up to 8 weeks. Specimens were analyzed by histological, enzyme histochemical, immunocytochemical, and ultrastructural methods and byin situ hybridization between day 7 and day 56 after seeding. In 56-day cultures, the MC3T3-E1 cells were arranged in a three-dimensional network and formation of bone-like tissue was indicated by calcification of a newly synthesized collagen type I matrix resembling osteoid and surrounding osteocyte-like cells. The differentiating culture showed high expression of osteocalcin and alkaline phosphatase activity. NIH3T3 fibroblasts used as control cells passed through the network of the substrate forming a confluent monolayer underneath. This culture system offers a potentially powerful model for bone formationin vitro and for investigating the osteogenic potential of bone-derived cells.

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Authors and Affiliations

  1. Institut für Pathologie, Gesellschaft für Strahlen- und Umweltforschung (GSF), Neuherberg, Federal Republic of Germany

    A. B. Murray

  2. Abteilung für Molekulare Zellpathologie, Gesellschaft für Strahlen-und Umwelt forschung, D-8042, Neuherberg, FRG

    M. Casser-Bette, E. I. Closs, V. Erfle &  J. Schmidt

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  1. M. Casser-Bette
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  2. A. B. Murray
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  3. E. I. Closs
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  4. V. Erfle
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  5. J. Schmidt
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Casser-Bette, M., Murray, A.B., Closs, E.I. et al. Bone formation by osteoblast-like cells in a three-dimensional cell culture. Calcif Tissue Int 46, 46–56 (1990). https://doi.org/10.1007/BF02555824

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  • DOI: https://doi.org/10.1007/BF02555824

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