Abstract
Thirty male Sprague-Dawley rats received weekly injections of dimethylhydrazine for three months. They were subsequently laparotomized and tumors were palpated in 21 rats. The colonic tumor was removed and a fraction was autotransplanted into the wall of the transverse colon and a second fraction into the subcapsular space of the left kidney. The results demonstrated successful autotransplantation of colonic tumors in 13 of 21 rats (i.e., 62 percent). In 11 of the 21 rats (52 percent) the tumor grew in the left kidney. Of the various experimental methods proposed for the study of tumor metastases, the one described herein appears to be the first in which primary and secondary tumors were studied in the same individual, since dimethylhydrazine-treated rats usually develop more than one colonic tumor per individual. The model may be useful to monitor the effect of various drugs known to affect tumor growth, not only at the primary site, but also at the site of metastasis.
Similar content being viewed by others
References
Ketcham AS, Wexler H, Minton JP. Experimental study of metastases. JAMA 1966;198:157–64
Mareel MM. Invasionin vitro: methods of analysis. Cancer Metastasis Rev 1983;2:201–18.
Martin MS, Martin F, Justrabo E, Michel MF, Lagneau A. Immunoprophylaxis and therapy of grafted rat colonic carcinoma. Gut 1977;18:232–5.
Weese JL, Gilbertson EM, Syrjala SE, Whitney PD, Starling JR. Reduced incidence of rat colon cancer metastases by perioperative immunostimulation with maleic anhydride-divinyl ether-2 (MVE-2). Dis Colon Rectum 1985;28:217–20.
Weiss L, Suh OW. The quantitation of some aspects of invasionin vivo andin vitro. In: Torisu M, Yoshida T. Basic mechanisms and clinical treatment of tumor metastasis. New York: Academic Press, 1985:24–39.
Mork S, De Ridder L, Laerum OD. Invasive pattern and phenotypic properties of malignant neurogenic rat cellsin vivo andin vitro. Anticancer Res 1982;2:1–10.
Schmidt M, Deschner EE, Thaler HT, Clements L, Good RA. Gastrointestinal cancer studies in the human to nude mouse heterotransplant system. Gastroenterology 1977;72:829–37.
Rubio CA, Nylander G, Wahlin B, Sveander M, Duvander A, Alun ML. Minimal invasive carcinoma of the colon in rats. Am J Pathol 1986;123:161–5.
Distelmans W, Van Ginckel R, Vanherck W, De Brabander M. The kidney invasion test: an assay following macroscopic quantification of malignant invasionin vivo. Invasion Metastasis 1985;5:170–84.
Van Ginckel R, Vanherck W, Willebrords R, Distelmans W, De Brabander M. The kidney invasion test: its application to the Lewis lung carcinoma system. Anticancer Res 1986;6:705–8.
Rubio CA, Nylander G, Wahlin B, Sveander M, Duvander A, Alun ML. Monitoring the histogenesis of colonic tumors in the Sprague-Dawley rat. J Surg Oncol 1986;31:225–6.
Rubio CA. Further studies on the therapeutic effect of indomethacin on esophageal tumors. Cancer 1986;58:1029–31.
Pollard M, Luckert PH. Treatment of chemical induced intestinal cancers with indomethacin. Proc. Soc. Exp Biol Med 1981;167: 161–4.
Author information
Authors and Affiliations
Additional information
This study was supported by grants from the Cancer Society of Stockholm and the Karolinska Institute.
About this article
Cite this article
Rubio, C.A., Wallin, B., Sveander, M. et al. Research. Dis Colon Rectum 30, 884–887 (1987). https://doi.org/10.1007/BF02555430
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF02555430