Skip to main content
SpringerLink
Log in

Two bovine models of osteogenesis imperfecta exhibit decreased apatite crystal size

  • Laboratory Investigations
  • Published:
Calcified Tissue International Aims and scope Submit manuscript

Summary

In recent years advances have been made in detailing the changes in both collagen and noncollagenous proteins caused by a variety of mutations leading to osteogenesis imperfecta. Much less, however, is known about the mineral phase in the affected bone. In this report, we measured the crystallinity of the apatite in bovine OI bone. Line broadening of the 002 reflection (which estimates changes in the long orc axis of the crystals) and of the 310 reflection (which estimates changes in the thickness of the crystals) both show large decreases (30 and 35% respectively). Transmission electron micrograph measurements indicate that these changes were most probably a result of smaller crystals. No decrease in the ash weight of the bone was observed.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. Sillence D (1981) Osteogenesis imperfecta: an expanding panorama of variants. Clin Orthop Rel Res 156:11–25

    Google Scholar 

  2. Byers PH, Shapiro JR, Rowe D, David KE (1983) Abnormal α-2 chain in type I collagen from a patient with a form of osteogenesis imperfecta. J Clin Invest 71:689–697

    Article  PubMed  CAS  Google Scholar 

  3. Chu ML, Williams CJ, Pepe G, Hirsch JC, Prockop DJ, Ramirez F (1983) Internal deletion in a collagen gene in a perinatal lethal form of osteogenesis imperfecta. Nature 304:78–80

    Article  PubMed  CAS  Google Scholar 

  4. de Wet WJ, Pihlajaniemi T, Myers J, Kelly TE, Prockop DJ (1983) Synthesis of a shortened pro α1 (I) chain and decreased synthesis of pro α2(I) chains in a pro band and with osteogenesis imperfecta. J Biol Chem 258:7721–7728

    PubMed  Google Scholar 

  5. Steinmann B, Rao VH, Vogel A, Bruckner P, Gitzelmann R, Byers PH, (1984) Cysteine in the triple-helical domain of one allelic product of the alpha I (I) gene of type I collagen produces a lethal form of osteogenesis imperfecta. J Biol Chem 259:11129–11138

    PubMed  CAS  Google Scholar 

  6. Dickson LA, Pihlajaniemi T, Deak SB, Pope MF, Nicholls AC, Prockop DJ, Myers JC (1984) Nuclease S1 mapping of a homozygous mutation in the carboxyl-propeptide coding region of the pro α2 (I) collagen gene in a patient with osteogenesis imperfecta. Proc Natl Acad Sci USA 81:4524–4528

    Article  PubMed  CAS  Google Scholar 

  7. Minor RR, Pihlajaniemi T, Prockop DJ, Denholm LJ, Wootton JAM (1984) Post-translational over-modification of type I, type III and type V collagen in bovine and human osteogenesis imperfecta. J Cell Biol 99:405a

    Google Scholar 

  8. Takagi Y, Veis A, Sauk JJ (1983) Relation of mineralized defects in collagen matrices to noncollagenous protein components. Clin Orthop Rel Res 176:282–290

    CAS  Google Scholar 

  9. Dickson IR, Millar EA, Veis A (1975) Evidence for abnormality of bone matrix proteins in osteogenesis imperfecta. Lancet 2:586–587

    Article  PubMed  CAS  Google Scholar 

  10. Dickson IR, Bagga M, Paterson CR (1983) Variations in the serum concentration and urine excretion of α1HS-glycoprotein, a bone-related protein in normal individuals and in patients with osteogenesis imperfecta. Calcif Tissue Int 35:16–20

    Article  PubMed  CAS  Google Scholar 

  11. Denholm LJ, Cole WG (1983) Heritable bone fragility, joint laxity and dysplastic dentin in Friesian calves: a bovine syndrome of osteogenesis imperfecta. Aust Vet J 60:9–17

    PubMed  CAS  Google Scholar 

  12. Fisher LW, Denholm LJ, Conn KM, Termine JD (1986) Mineralized tissue protein profiles in the Australian form of bovine osteogenesis imperfecta. Calcif Tissue Int 38:16–20

    PubMed  CAS  Google Scholar 

  13. Termine JD, Gehron Robey P, Fisher LW, Shimokawa H, Drum MA, Hawkins GR, Cruz JB, Thompson KG (1984) Osteonectin, bone proteoglycan, and phosphophoryn defects in a form of bovine osteogenesis imperfecta. Proc Natl Acad Sci USA 81:2213–2217

    Article  PubMed  CAS  Google Scholar 

  14. Klug HP, Alexander LE (1962) X-ray diffraction procedures. John Wiley and Sons, New York, pp 491–538

    Google Scholar 

  15. Spurr AR (1969) A low-viscosity resin embedding medium for electron microscopy. J Ultrastruct Res 41:115–132

    Google Scholar 

  16. Shupe JL, Eanes ED, Leone NC (1981) Effect of excessive exposure to sodium fluoride on composition and crystallinity of equine bone tumors. Am J Vet Res 42:1040–1042

    PubMed  CAS  Google Scholar 

  17. Posner AS, Eanes ED, Harper RA, Zipkin I (1963) X-ray diffraction analysis of the effect of fluoride on human bone apatite. Arch Oral Biol 8:549–570

    Article  Google Scholar 

  18. Zipkin I, Eanes ED, Shupe JL (1964) Effect of prolonged exposure to fluoride on the ash, fluoride, citrate, and crystallinity of bovine bone. Am J Vet Res 25:1595–1597

    CAS  Google Scholar 

  19. Eanes ED, Zipkin I, Harper RA, Posner AS (1965) Smallangle X-ray diffraction analysis of the effect of fluoride on human bone apatite. Arch Oral Biol 10:161–173

    Article  PubMed  CAS  Google Scholar 

  20. Eanes ED, Termine JD (1983) Calcium in mineralized tissues. In: Spiro TG (ed) Calcium in biology. John Wiley & Sons, New York, pp 201–233

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Bone Research Branch, National Institute of Dental Research, National Institutes of Health, 20892, Bethesda, Maryland, USA

    L. W. Fisher, E. D. Eanes, L. J. Denholm, B. R. Heywood & J. D. Termine

Authors
  1. L. W. Fisher
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. E. D. Eanes
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. L. J. Denholm
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. B. R. Heywood
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. J. D. Termine
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Fisher, L.W., Eanes, E.D., Denholm, L.J. et al. Two bovine models of osteogenesis imperfecta exhibit decreased apatite crystal size. Calcif Tissue Int 40, 282–285 (1987). https://doi.org/10.1007/BF02555262

Download citation

  • Received:

  • Revised:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF02555262

Key words

Search

Navigation