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Influence of 1,25-dihydroxyvitamin D3 on cell proliferation during odontogenesis of the mouse embryonic molarsIn vitro

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Summary

Mandibular first molars from 17-day-old mouse embryos were cultured for 2 and 4 days in alpha-MEM supplemented with 10% newborn bovine serum containing 0.02% ethanol or 0.1, 1.0 or 10 ng/ml 1,25-(OH)2D3. After embedding, every 6th section was stained and mitotic features of inner dental epithelium (IDE) and dental papilla cells (DP) were counted under a light microscope. On the 2nd day, no significant differences were observed in the IDE and DP mitotic indexes among the control, ethanol, and the three vitamin D groups. On the 4th day, the indexes for all groups decreased. But the IDE indexes for all vitamin D groups were significantly different from those for the control (P<0.01) and ethanol (P<0.05) groups, whereas the DP index was significant (P<0.05) only in the 10 ng/ml vitamin D group. The present results suggest that vitamin D3 may have an influence on cell proliferation of IDE and DP in mouse embryonic molarsin vitro.

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Authors and Affiliations

  1. Department of Oral Anatomy, School of Dentistry, Iwate Medical University, 020, Morioka, Japan

    Yasunori Sakakura, Naoki Fujiwara, Kiyoto Ishizeki & Tokio Nawa

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  1. Yasunori Sakakura
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  2. Naoki Fujiwara
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  3. Kiyoto Ishizeki
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  4. Tokio Nawa
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Sakakura, Y., Fujiwara, N., Ishizeki, K. et al. Influence of 1,25-dihydroxyvitamin D3 on cell proliferation during odontogenesis of the mouse embryonic molarsIn vitro . Calcif Tissue Int 43, 46–49 (1988). https://doi.org/10.1007/BF02555168

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  • DOI: https://doi.org/10.1007/BF02555168

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