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Crystal-collagen relationships in calcified turkey leg tendons visualized by selected-area dark field electron microscopy

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Summary

The distribution and orientation of biological apatite crystals in calcified turkey leg tendons were studied by selected-area dark field electron microscopy. This imaging technique enables the direct visualization of apatite and the specific determination of the crystallographic axes (a, b-axes or c-axis) within calcified collagen fibrils. This study shows that at early stages of mineralization, rod-shaped apatite crystals (5–20 nm in length) were localized and dispersed within gap zones bordering both the collagen molecule C- and N-terminal regions. At later stages of mineral deposition the crystals were more extensive, occupying greater areas of the gap zone and, in addition, apatite crystals were found to occur in the overlap zones. The orientation of apatite crystals was observed to be an alternating and interlocking distribution of a, b-axes and c-axis along the axial period of collagen fibrils. This distribution is interpreted as representing a continuous rotation of apatite axial orientation along the collagen period.

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References

  1. Robinson RA, Watson ML (1952) Collagen-crystal relationships in bone as seen in the electron microscope. Anat Rec 114:383–410

    Article  PubMed  CAS  Google Scholar 

  2. Nylen MU, Scott DB, Mosley VM (1960) Mineralization of turkey leg tendon. II. Collagen-mineral relations revealed by electron and X-ray microscopy. In: Sognnaes RF (ed) Calcification in biological systems. American Association for the Advancement of Science, Washington DC, pp 129–142.

    Google Scholar 

  3. Myers HM, Engström A (1965) A note on the organization of hydroxyapatite in calcified tendons. Exp Cell Res 40:182–185

    Article  PubMed  CAS  Google Scholar 

  4. Glimcher MJ, Krane SM (1968) The organization and structure of bone, and the mechanism of calcification. In: Gould BS (ed) Treatise on collagen. IIB. Academic Press, New York, pp 68–251

    Google Scholar 

  5. Höhling HJ, Kreilos R, Neubauer G, Boyde A (1971) Electron microscopy and electron microscopical measurements of collagen mineralization in hard tissues. Z Zellforsch 122:36–52

    Article  PubMed  Google Scholar 

  6. Landis WJ (1986) A study of calcification in the leg tendons from the domestic turkey. J Ultrastruct Mol Struct Res 94:217–238

    Article  PubMed  CAS  Google Scholar 

  7. Engström A (1966) Apatite-collagen organization in calcified tendon Exp Cell Res 43:241–245

    Article  PubMed  Google Scholar 

  8. Eanes ED, Lundy DR, Martin GN (1970) X-ray diffraction study of the mineralization of turkey leg tendon. Calcif Tissue Res 6:239–248

    Article  PubMed  CAS  Google Scholar 

  9. Lundy DR, Eanes ED (1973) An x-ray line-broadening study of turkey leg tendon. Archs Oral Biol 18:813–826

    Article  CAS  Google Scholar 

  10. Ascenzi A, Bonucci E, Ripamonti A, Roveri N (1978) X-ray diffraction and electron microscope study of osteons during calcification. Calcif Tissue Res 25:133–143

    Article  PubMed  CAS  Google Scholar 

  11. White SW, Hulmes DJS, Miller A, Timmins PA (1977) Collagen-mineral axial relationship in calcified turkey leg tendon by x-ray and neutron diffraction. Nature 266:421–425

    Article  PubMed  CAS  Google Scholar 

  12. Berthet-Colominas C, Miller A, White SW (1979) Structural study of the calcifying collagen in turkey leg tendons. J Mol Biol 134:431–445

    Article  PubMed  CAS  Google Scholar 

  13. Pease DC (1966) Anhydrous ultrathin sectioning and staining for electron microscopy. J Ultrastruct Res 14:379–390

    Article  PubMed  CAS  Google Scholar 

  14. Arsenault AL, Grynpas MD (1988) Crystals in calcified epiphyseal cartilage and cortical bone of the rat. Calcif Tissue Int 43:219–225

    PubMed  CAS  Google Scholar 

  15. Arsenault AL, Hunziker EB (1988) Electron microscopic analysis of mineral deposits in the calcifying epiphyseal growth plate. Calcif Tissue Int 42:119–126

    PubMed  CAS  Google Scholar 

  16. Hodge AJ, Schmitt FO (1960) The charge profile of the tropocollagen macromolecule and the packing arrangement in native-type collagen fibrils. Proc Natl Acad Sci USA 46:186–197

    Article  PubMed  CAS  Google Scholar 

  17. Chapman JA (1974) The staining patterns of collagen fibrils. I. An analysis of electron micrographs. Connect Tissue Res 2:137–150

    Article  PubMed  CAS  Google Scholar 

  18. Chapman JA, Hardcastle RA (1974) The staining pattern of collagen fibrils. II. A comparison with patterns computergenerated from the amino acid sequence. Connect Tissue Res 2:151–159

    PubMed  CAS  Google Scholar 

  19. Meek KM, Chapman JA, Hardcastle RA (1979) The staining pattern of collagen fibrils. Improved correlation with sequence data. J Biol Chem 254:10710–10714

    PubMed  CAS  Google Scholar 

  20. Mineral Powder Diffraction File Compiled by the Joint Committee on Powder Diffraction Standards Data Book, 1980, vol 1, Swarthmore, Pennsylvania 447

  21. Hulmes DJS, Miller A (1979) Quasi-hexagonal molecular packing in collagen fibrils. Nature 282:878–890

    Article  PubMed  CAS  Google Scholar 

  22. Hulmes DJS, Holmes DF, Cummings C (1985) Crystalline regions in collagen fibrils. J Mol Biol 184:473–477

    Article  PubMed  CAS  Google Scholar 

  23. Arsenault AL, Ottensmeyer FP (1983) Quantitative spatial distributions of calcium, phosphorus, and sulfur in calcifying epiphysis by high resolution electron spectroscopic imaging. Proc Natl Acad Sci USA 80:1322–1326

    Article  PubMed  CAS  Google Scholar 

  24. Arsenault AL, Ottensmeyer FP (1984) Visualization of early intramembranous ossification by electron microscopic and spectroscopic imaging. J Cell Biol 98:511–521

    Article  Google Scholar 

  25. Miller A (1984) Collagen: the organic matrix of bone. Phil Trans R Soc Lond B 304:455–477

    CAS  Google Scholar 

  26. Glimcher MJ, Brickley-Parson D, Kossiva D (1979) Phosphopeptides and γ-glutamic acid-containing peptides in calcified turkey tendon: their absence in uncalcified tendon Calcif Tissue Int 27:281–284

    Article  PubMed  CAS  Google Scholar 

  27. Hauschka PV, Lian JB, Gallop PM (1975) Direct identification of the calcium-binding amino acid γ-carboxyglutamate in mineralized tissue. Proc Natl Acad Sci USA 72:3925–3929

    Article  PubMed  CAS  Google Scholar 

  28. Boskey AL, Posner AS (1977) The role of synthetic and bone-extracted Ca-phospholipid-phosphate complexes in hydroxyapatite formation. Calcif Tissue Res 23:251–258

    Article  PubMed  CAS  Google Scholar 

  29. Hinek A, Reiner A, Poole AR (1987) The calcification of cartilage matrix in chondrocyte culture: studies of the C-propeptide of type II collagen (chondrocalcin). J Cell Biol 104:1435–1441

    Article  PubMed  CAS  Google Scholar 

  30. Arsenault AL, Ottensmeyer FP, Heath BI (1988) An electron microscopic and spectroscopic study of epiphyseal cartilage: analysis of fine structure and matrix vesicles preserved by freeze substitution. J Ultrastruct Mol Struct Res 98:32–47

    Article  PubMed  CAS  Google Scholar 

  31. Arsenault AL, Spitzer E, Simon GT (1987) Improved preservation of cartilage extracellular matrix by freeze-dried embedding. J Microsc 145:357–360

    PubMed  CAS  Google Scholar 

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  1. Department of Anatomy, University of British Columbia, 2177 Wesbrook Mall, V6T 1W5, Vancouver, British Columbia, Canada

    A. Larry Arsenault

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  1. A. Larry Arsenault
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Arsenault, A.L. Crystal-collagen relationships in calcified turkey leg tendons visualized by selected-area dark field electron microscopy. Calcif Tissue Int 43, 202–212 (1988). https://doi.org/10.1007/BF02555136

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  • DOI: https://doi.org/10.1007/BF02555136

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