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Insulin-like growth factor-I and insulin-like growth factor-II induce c-fos in mouse osteoblastic cells

  • Molecular and Cellular Biology
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Summary

We investigated the expression of c-fos in mouse osteoblast-like cultures treated with insulin-like growth factor (IGF)-I and IGF-II. The IGFs are present in bone, are produced by osteoblast-like cells in culture, and stimulate osteoblast cell proliferation. Quiescent, subconfluent cultures of the clonal osteoblast-like mouse calvarial cell line, MC3T3-E1, were treated with 10 ng/ml of IGF-I or IGF-II. RNA was extracted at 0, 15, 30, 60, 120 and 240 minutes, and c-fos messenger RNA (mRNA) was analyzed on Northern blots. Both IGFs transiently increased c-fos mRNA levels 25–28 fold at 15–30 min. To determine if c-fos induction was unique to the MC3T3-E1 cell line, effects of IGF-1 and IGF-II (3 ng/ml) were also tested in quiescent, serum-free primary mouse calvarial cells. Levels of c-fos mRNA were increased at 15 and 30 minutes (40-fold with IGF-I and 5-fold with IGF-II). These results indicate that IGF-I and IGF-II caused a rapid and transient induction of c-fos mRNA in murine osteoblasts.

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References

  1. Van Straaten F, Mueller R, Curran T, Van Beveren C, Verma IM (1983) Complete nucleotide sequence of a human c-onc gene: deduced amino acid sequence of the human c-fos protein. Proc Natl Acad Sci USA 80:3183–3187

    Article  PubMed  Google Scholar 

  2. Mueller R, Bravo R, Burckhardt J, Curran T (1984) Induction of c-fos gene and protein by growth factors precedes activation of c-myc. Nature 312:716–720.

    Article  Google Scholar 

  3. Mueller R, Wagner EF (1984) Differentiation of F9 teratocarcinoma stem cells after transfer of c-fos proto-oncogenes. Nature 311:438–442

    Article  CAS  Google Scholar 

  4. Sassone-Corsi P, Ransone LJ, Lamph WW, Verma IM (1988) Direct interaction between fos and jun nuclear oncoproteins: role of the ‘leucine zipper’ domain. Nature 336:692–695

    Article  PubMed  CAS  Google Scholar 

  5. Gentz R, Rauscher FJ III, Abate C, Curran T (1989) Parallel association of fos and jun leucine zippers juxtaposes DNA binding domains. Science 243:1695–1699

    Article  PubMed  CAS  Google Scholar 

  6. Curran T, Teich NM (1982) Candidate product of the FBJ murine osteosarcoma virus oncogene: characterization of a 55,000-dalton phosphoprotein. J. Virol 42:114–122

    PubMed  CAS  Google Scholar 

  7. Verma IM, Graham RG (1987) The fos oncogene. Adv Cancer Res 49:29–52

    PubMed  CAS  Google Scholar 

  8. Ruether U, Garber C, Komitowski D, Mueller R, Wagner EF (1987) Deregulated c-fos expression interferes with normal bone development in transgenic mice. Nature 325:412–416

    Article  CAS  Google Scholar 

  9. Mueller R, Slamon DJ, Tremblay JM, Cline MJ, Verma IM (1982) Differential expression of cellular oncogenes during pre-and postnatal development of the mouse. Nature 299:640–644

    Article  Google Scholar 

  10. Greenberg ME, Ziff EB (1984) Stimulation of 3T3 cells induces transcription of the c-fos proto-oncogene. Nature 311:433–438

    Article  PubMed  CAS  Google Scholar 

  11. Mohan S, Linkhart T, Farley J, Baylink D (1984) Bone derived factors active on bone cells. Cacif Tissue Int 36:S139-S149

    Article  Google Scholar 

  12. Canalis E, McCarthy T, Centrella (1988) Growth factors and the regulation of bone remodeling. J Clin Invest 81:277–281

    Article  PubMed  CAS  Google Scholar 

  13. Raisz LG (1988) Local and systemic factors in the pathogenesis of osteoprosis. N Engl J Med 318:818–828

    Article  PubMed  CAS  Google Scholar 

  14. Mueller R, Mueller D, Guilbert L (1984) Differential expression of c-fos in hematopoietic cells: correlation with differentiation of monomyelocytic cells in vitro. EMBO J 3:1887–1890

    CAS  Google Scholar 

  15. Mohan S, Wergedal JE, Taylor A, Baylink D (1988) IGF-I and II are produced by human osteoblast-like cells in culture (Abstr). FASEB J 2(4):842

    Google Scholar 

  16. Mohan S, Linkhart TA, Jennings JC, Baylink DJ (1988) Identification and quantification of four distinct growth factors stored in human bone matrix (Abstr). Transactions of the 34th annual meeting of the orthopaedic research society, Atlanta. 13:322 (Feb 1–4)

    Google Scholar 

  17. Mohan S, Jennings JC, Linkhart TA, Baylink DJ (1988) Primary structure of human skeletal growth factor: homology with human insulin-like growth factor-II. Biochem Biophys Acta 966:44–55

    PubMed  CAS  Google Scholar 

  18. Linkhart TA, Jennings JC, Mohan S, Wakley GK, Baylink DJ (1986) Characterization of mitogenic activities extracted from bovine bone matrix. Bone 7:479–487

    Article  PubMed  CAS  Google Scholar 

  19. Kodama H, Amagai Y, Sudo H, Kasai S, Yamamoto S (1981) Establishment of a clonal osteogenic cell line from newborn mouse calvaria. Jpn J Oral Biol 23:899–901

    Google Scholar 

  20. Chirgwin JM, Przybyla AE, MacDonald RJ, Rutter WJ (1979) Isolation of biologically active ribonucleic acid from sources enriched in ribonuclease. Biochemistry 18:5294–5299

    Article  PubMed  CAS  Google Scholar 

  21. Lehrach H, Diamond D, Wozney JM, Boedtker H (1977) RNA molecular weight determinations by gel electrophoresis under denaturing conditions, a critical reexamination. Biochemistry 16:4743–4751

    Article  PubMed  CAS  Google Scholar 

  22. Schleicher and Schuell (1987) Transfer and immobilization of nucleic acids to 545 solid supports. Schleicher and Schuell technical bulletin no. 700, Keene, NH

    Google Scholar 

  23. Feinberg AP, Vogelstein B (1983) A technique for radiolabeling DNA restriction endonuclease fragments to high specific activity. Anal Biochem 132:6–13

    Article  PubMed  CAS  Google Scholar 

  24. Herrin DL, Schmidt GW (1988) Rapid, reversible staining of northern blots prior to hybridization. Bio Techniques 6:196–200

    CAS  Google Scholar 

  25. Merriman H, Mohan S, Linkhart T, Linkhart S, Baylink D (1986) Evidence that a skeletal growth factor-like (SGF-like) factor is an autocrine effector of osteoblast collagen synthesis (Abstr). J Bone Min Res 1 (supl 1):455

    Google Scholar 

  26. Linkhart TA, Mohan S (1989) Parathyroid hormone stimulates release of insulin-like growth factor-I (IGF-I) and IGF-II from neonatal mouse calvaria in organ culture. Endocrinology 125:1484–1491

    PubMed  CAS  Google Scholar 

  27. Ong J, Yamashita S, Melmed S (1987) IGF-I induces c-fos messenger ribonucleic acid in L6 rat skeletal muscle cells. Endocrinology 120:353–357

    Article  PubMed  CAS  Google Scholar 

  28. Damante G, Cox F, Rapoport B (1988) IGF-I increases c-fos expression in FRTL5 rat thyroid cells by activating the c-fos promoter. Biochem Biophys Res Commun 151:1194–1199

    Article  PubMed  CAS  Google Scholar 

  29. Nishikura K, Murray JM (1987) Antisense RNA of protooncogene c-fos blocks renewed growth of quiescent 3T3 cells. Mol Cell Biol 7:639–649

    PubMed  CAS  Google Scholar 

  30. Verma I, Mitchell RL, Kruijer W, Van Beveren C, Zokas L, Hunter T, Cooper JA (1985) Proto-oncogene fos: induction and regulation during growth and differentiation. In: Cancer Cells 3/growth and differentiation. Feramisco J, Ozanne B, Skiles C (eds) Cold Spring Harbor Laboratory, Cold Spring Harbor, NY, pp 275–319

    Google Scholar 

  31. Strong DD, Wergedal JE, Beachler AL, LaTour DA, Mohan S, Linkhart TA, Baylink DJ (1988) Skeletal growth factor and transforming growth factor beta-1 enhance expression of type I procollagen mRNA in human osteoblast-like cells (Abstr). Program and abstracts of the 70th annual meeting of the endocrine society, New Orleans, p 869

  32. McCarthy TL, Centrella M, Canalis E (1989) Regulatory effects of insulin-like growth factors I and II on bone collagen synthesis in rat calvarial cultures. Endocrinology 124:301–309

    PubMed  CAS  Google Scholar 

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Authors and Affiliations

  1. Department of Biochemistry, Loma Linda University and Jerry L. Pettis Veterans Administration Hospital, 11201 Benton Street, Loma Linda, California, USA

    Harold L. Merriman, Donn La Tour, Thomas A. Linkhart, Subburaman Mohan, David J. Baylink & Donna D. Strong Ph.D.

  2. Department of Medicine, Loma Linda University and Jerry L. Pettis Veterans Administration Hospital, 11201 Benton Street, 92357, Loma Linda, California, USA

    Subburaman Mohan, David J. Baylink & Donna D. Strong Ph.D.

  3. Department of Pediatrics, Loma Linda University and Jerry L. Pettis Veterans Administration Hospital, 11201 Benton Street, 92357, Loma Linda, California, USA

    Thomas A. Linkhart

  4. Department of Physiology, Loma Linda University and Jerry L. Pettis Veterans Administration Hospital, 11201 Benton Street, 92357, Loma Linda, California, USA

    Subburaman Mohan

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  1. Harold L. Merriman
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  2. Donn La Tour
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  4. Subburaman Mohan
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  5. David J. Baylink
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  6. Donna D. Strong Ph.D.
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Merriman, H.L., La Tour, D., Linkhart, T.A. et al. Insulin-like growth factor-I and insulin-like growth factor-II induce c-fos in mouse osteoblastic cells. Calcif Tissue Int 46, 258–262 (1990). https://doi.org/10.1007/BF02555005

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  • DOI: https://doi.org/10.1007/BF02555005

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