Spermatozoa selection by the swim-up procedure and two-layer percoll gradient centrifugation

Abstract

The efficiency of the swim-up procedure and two-layer Percoll gradient centrifugation in procession of spermatozoa was assessed in ejaculates from 47 infertile men. A significantly higher total number of spermatozoa was harvested from Percoll gradients than from the swim-up procedure, the loss rates in concentration being −13.6±6.4% and −70.8±5.8%, respectively (p<0.0001). Recovery in per cent motility was significantly higher after the Percoll gradient than after the swim-up procedure (34.8±10.2% versus −10.4±17.2%, p<0.05). No significant difference was noted between the mean motility grades of the final solutions obtained by the two methods (2.7±0.2 and 2.0±0.4, respectively, p>0.05). When evaluation was conducted within three initial fresh sample concentration categories such as severe oligospermia (lower than 5×10⁶/ml), moderate oligospermia (5 to 10×10⁶/ml) and mild oligospermia (higher than 10×10⁶/ml), the Percoll technique recovered, significantly higher number of spermatozoa than the swim-up procedure through all concentration categories (p<0.05 for each range). Despite being statistically insignificant, Percoll gradients produced final spermatozoa pools with higher per cent motility and motility quality within all concentration ranges. The results suggest that the Percoll gradient centrifugation should be the preferred selection method regardless of the initial fresh sample concentration.