Abstract
Using monospecific antibodies to purified human apolipoproteins, immunofluorescence microscopy of renal biopsies from 4 patients with nephrotic syndrome revealed apolipoprotein (apo) AI, apo CIII and apo B (LDL) in lysosomes of the proximal tubular cells.
This supports the hypothesis that there is increased filtration of both high density lipoproteins (HDL) and low density lipoproteins (LDL) with partial reabsorption by the tubules, thus affecting the serum lipoprotein levels.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Segal, P., Gidex, L. I., Vega, G. L., Edelstein, D., Eder, H. A., Roheim, P. S.: Apoproteins of high density lipoproteins in the urine of normal subjects.J. Lipid Res., 20, 784 (1979).
Mendoza, S. G., Kashyap, M. L., Chen, C. Y., Lutmer, R. F.: High density lipoproteinuria in nephrotic syndrome.Metabolism, 25, 1143 (1976).
Kashyap, M. L., Ooi, B. S., Hynd, B. M., Glueck, C. J., Pollak, V. E., Robinson, K.: Sequestration and excretion of high density and low density lipoproteins by the kidney in human nephrotic syndrome.Artery, 6, 108 (1979).
Shore, V. G., Forte, T., Licht, H., Lewis, S. B.: Serum and urinary lipoproteins in the human nephrotic syndrome: Evidence for renal catabolism of lipoproteins.Metabolism, 31, 258 (1982).
Gherardi, E., Vecchia, L., Calandra, S.: Experimental nephrotic syndrome in the rat induced by puromycin aminonucleoside. Plasma and urinary lipoproteins.Exp. Molec. Path., 32, 128 (1980).
Radola, B. J.: Isoelectric focussing in layers of granulated gels.Biochim. Biophys. Acta, 386, 181 (1974).
Baker, H. N., Jackson, R. L., Gotto, A. M.: Isolation and characterization of the cyanogen bromide fragments from the high-density apolipoprotein glutamine I.Biochemistry, 12, 3866 (1973).
Saku, K., Gartside, P. S., Hynd, B. A., Kashyap, M. L.: Mechanism of action of gemfibrozil on lipoprotein metabolism.J. Clin. Invest., 75, 1702 (1985).
Saku, K., Ahmad, M., Glas-Grenwalt, P., Kashyap, M. L.: Activation of fibrinolysis by apolipoproteins in man.Thromb. Res. 39, 1 (1985).
Saku, K., Reddy, G. S., Hynd, B. A., Kashyap, M. L.: Renal handling of high density lipoproteins by isolated perfused kidneys.Metabolism, 33, 432 (1984).
Peterson, D. R., Hjelle, J. T., Carone, F. A., Moore, P. A.: Renal handling of plasma high density lipoprotein.Kidney Int., 26, 411 (1984).
Nakai, T., Whayne, T. F.: Catabolism of canine apolipoprotein A-I: purification, catabolic rate, organs of catabolism and the liver subcellular catabolic site.J. Lab. Clin. Med., 88, 63 (1976).
Glass, C. K., Pittman, R. C., Keller, G. A., Steinberg, D.: Tissue sites of degradation of apoprotein A-I in the rat.J. Biol. Chem., 258, 7161 (1983).
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Saku, K., Sata, T., Naito, S. et al. Apolipoproteins in human biopsied nephrotic kidneys. International Urology and Nephrology 20, 429–438 (1988). https://doi.org/10.1007/BF02549577
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF02549577