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Renal tubular epithelial antigen-containing immune complexes stimulate interleukin-1 production by monocytes from patients with glomerulonephritis

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Abstract

We have investigated the effect of immune complexes (IC) derived from human renal tubular epithelial (RTE) antigen on the release of interleukin-1 (IL-1) in monocyte cultures from patients with glomerulonephritis (GN). When peripheral blood monocytes (PBM) were activated by IC, substantial amounts of IL-1 could be detected in the supernatants as measured by mouse thymocyte assay. The IC-induced IL-1 activity was significantly higher in patients with GN than in normal controls. To avoid the effect of prostaglandins on the IL-1 assay, we cultured PBM with addition of indomethacin and assayed IL-1 activity in the culture supernatants. This cyclooxygenase inhibitor augmented IC-induced IL-1 production. The results suggest that IC are involved in stimulating IL-1 production by PBM and thus play a role in the immune response in GN.

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  1. Second Department of Internal Medicine, Nihon University School of Medicine, Tokyo, Japan

    K. Matsumoto

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  1. K. Matsumoto
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Matsumoto, K. Renal tubular epithelial antigen-containing immune complexes stimulate interleukin-1 production by monocytes from patients with glomerulonephritis. International Urology and Nephrology 24, 319–326 (1992). https://doi.org/10.1007/BF02549542

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