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Calorimetric studies of thermal denaturation of DNA and tRNAs

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Abstract

The thermal denaturation of DNA and tRNAs was investigated by differential scanning calorimetry (DSC). Endothermic peaks were observed in the DSC curves when the samples were heated. The denaturation temperatures (T d) and denaturation enthalpies (ΔH d) of samples of lyophilized powder and of samples in 4 mol/dm3 urea solution were obtained. The experimental results showed that the thermal stabilities of DNA and tRNAs were obviously different under these conditions.

Zusammenfassung

Mittels DSC wurde die thermische Denaturierung von DNA und tRNA untersucht. Beim Erhitzen der Proben werden an den DSC-Kurven endotherme Peaks festgestellt. Temperature (T d) und Enthalpie (ΔH d) der Denaturierung der Proben von liofilisiertem Pulver und von Proben in einer 4 mol/dm3 Harnstofflösung wurden ermittelt. Die experimentellen Ergebnisse zeigen, daß sich die thermische Stabilität von DNA und tRNA unter diesen Umständen eindeutig unterscheidet.

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Authors and Affiliations

  1. Institute of Chemistry, Academia Sinica, 100080, Beijing, P. R. China

    Yunna Liu & Fu Tan

Authors
  1. Yunna Liu
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  2. Fu Tan
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Additional information

This research was supported by the National Science Foundation of China.

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Liu, Y., Tan, F. Calorimetric studies of thermal denaturation of DNA and tRNAs. Journal of Thermal Analysis 45, 35–38 (1995). https://doi.org/10.1007/BF02548660

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  • DOI: https://doi.org/10.1007/BF02548660

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