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Analysis of developing enamel of the rat

III. Carbohydrate, DEAE-sephadex, and immunological studies

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Abstract

Carbohydrate analyses showed L-fucose, D-xylose, D-mannose, D-arabinose, N-acetyl glucosamine, N-acetyl galactosamine, N-acetyl mannosamine, glucuronic acid and several unknown species to be present in the immature enamel or in one of its fractions. The concentration of carbohydrates was greatest in the water-extractable fraction and next greatest in the water-insoluble fraction. The water-extractable, EDTA-water-soluble, and water-insol-uble fractions each differed in their spectrum of carbohydrates. With DEAE-Sephadex fractionation at pH 5.5, the water-extractable fraction was separated partially into 7 peaks. Four fractions were recovered from the 7 peaks, each of which differed from the others in amino acid and carbohydrate content and in polyacrylamide gel characteristics. Immuno-electrophoretic procedures showed that antibodies will develop against the EDTA-water-soluble fraction.

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Authors and Affiliations

  1. Departments of Anatomy and Oral Biology, University of Kentucky, Medical Center, 40506, Lexington, Kentucky, USA

    W. K. Elwood

  2. Department of Preventive Dentistry, College of Dentistry, University of Athens, Athens, Greece

    A. X. Apostolopoulos

Authors
  1. W. K. Elwood
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  2. A. X. Apostolopoulos
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Elwood, W.K., Apostolopoulos, A.X. Analysis of developing enamel of the rat. Calc. Tis Res. 17, 337–347 (1975). https://doi.org/10.1007/BF02546605

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  • DOI: https://doi.org/10.1007/BF02546605

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