Abstract
Using radioactive substrate, thin-layer chromatography and recrystallization methods as well as differential centrifugation, gel filtration and electrophoresis, testosterone metabolism was investigated in male rat epiphyseal growth plate. 5α-androstane 3α-17β-diol was found to be a major metabolitein vitro; lesser amounts of androstenedione and androstanedione, and a very small amount of 5α-dihydrotestosterone were also identified. Radioactivity was recovered in epiphyseal subcellular fractions 30 minutes followingin vivo administration of tritiated 5α-dihydrotestosterone; within 2 hours radioactivity had fallen to essentially background level in all fractions but the cytosol. A testosterone binding protein could not be identified within epiphyseal cytosol.
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Axelrod, L. R., Matthijssen, C., Goldzieher, J. W., Pulliam, J. E.: Definitive identification of microquantities of radioactive steroids by recrystallization to constant specific activity. Acta endocr. (Kbh.), Suppl.99, 7–66 (1965)
Bayley, N., Gordon, G. S., Lisser, H.: Long-term experiences with methyltestosterone as a growth stimulant in short immature boys. Pediat. Clin. N. Amer.4, 819–825 (1957)
Blaquier, J. A., Calandra, R. S.: Intranuclear receptor for androgens in rat epididymis. Endocrinology93, 51–60 (1973)
Bruchovsky, N., Wilson, J. D.: The conversion of testosterone to 5α-androstan-17β-ol-3-one by rat prostatein vivo andin vitro. J. biol. Chem.243, 2012–2021 (1968)
Davis, B. J.: Disc electrophoresis-II. Method and application to human serum proteins. Ann. N. Y. Acad. Sci.121, 404–427 (1964)
Jaffe, R. B.: Testosterone metabolism in target tissues: hypothalamic and pituitary tissues of the adult rat and human fetus, and the immature rat epiphysis. Steroids14, 483–498 (1969)
Lasnitzki, I., Franklin, H. R., Wilson, J. D.: The mechanism of androgen uptake and concentration by rat ventral prostate in organ culture. J. Endocr.60, 81–90 (1974)
Mainwaring, W. I. P.: The binding of [1,2−3H] testosterone within nuclei of the rat prostate. J. Endocr.44, 323–333 (1969a)
Mainwaring, W. I. P.: A soluble androgen receptor in the cytoplasm of rat prostate. J. J. Endocr.45, 531–541 (1969b)
Mainwaring, W. I. P.: The separation of androgen receptor and 5α-reductase activities in subcellular fractions of rat prostate. Biochem. biophys. Res. Commun.40, 192–198 (1970)
Mainwaring, W. I. P., Mangan, F. R.: A study of the androgen receptors in a variety of androgen-sensitive tissues. J. Endocr.59, 121–139 (1973)
Puche, R. C., Romano, M. C.: A time course study of the effects of testosterone on the femoral metaphyses of young mice. Calcif. Tiss. Res.7, 103–107 (1971)
Santi, D. V., Sibley, C. H., Perriard, E. R., Tomkins, G. M., Baxter, J. D.: A filter assay for steroid hormone receptors. Biochemistry12, 2412–2416 (1973)
Silberberg, M., Silberberg, R.: Further investigations on the effect of the male sex hormone on endochondral ossification. Anat. Rec.95, 97–117 (1946)
Sobel, E. H., Raymond, C. S., Quinn, K. V., Talbot, N. B.: The use of methyltestosterone to stimulate growth: relative influence on skeletal maturation and linear growth. J. clin. Endocr.16, 241–248 (1956)
Vittek, J., Altman, K., Gordon, G. G., Southren, A. L.: The metabolism of 7α-3H-testosterone by rat mandibular bone. Endocrinology94, 325–329 (1974)
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Ackerman, R.J., Hamilton, D.W. Testosterone metabolism in male rat epiphysis. Calc. Tis Res. 20, 31–40 (1976). https://doi.org/10.1007/BF02546395
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DOI: https://doi.org/10.1007/BF02546395