Abstract
Transesterification and alcoholysis reactions catalyzed by immobilized lipases fromMucor miehei andHumicola lanuginosa in hexane gave fatty acid esters that did not reflect the expected 1,3-specificity of the enzymes, due to competing acyl migrations in the partial glyceride products. However, both lipases were 1,3-specific in reactions when diethyl ether was used as a solvent, and this provided a convenient analytical methodology in combination with gas chromatography and nuclear magnetic resonance spectroscopy for the determination of fatty acid distribution within triglycerides.
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Goh, S.H., Yeong, S.K. & Wang, C.W. Transesterification of cocoa butter by fungal lipases: Effect of solvent on 1,3-specificity. J Am Oil Chem Soc 70, 567–570 (1993). https://doi.org/10.1007/BF02545320
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DOI: https://doi.org/10.1007/BF02545320