Abstract
Galactosyl-6-[3H] glucosyl ceramide was prepared by the sequential action of galactose oxidase and sodium borohydride-[3H] reduction. A water-soluble radioactive contaiminant appeared after a 2 month storage at −4 C. This was identified as lactose-[3H] by both chromatographic and carrier dilution techniques.
References
Hajra, A.K., D.M. Bowen, Y. Kishimoto and N.S. Radin, J. Lipid Res. 7: 379 (1966).
Radin, N.S., L. Hof, R. Bradley and R.O. Brady, Brain Res. 14: 497 (1969).
Suzuki, K., and Y. Suzuki, Proc. Nat. Acad. Sci. (U.S.) 66: 302 (1970).
Dawson, G., and A. Stein, Science 170: 557 (1970).
Bradley, R.M., and J.N. Kanfer, Biochim. Biophys. Acta 84: 210 (1964).
Suzuki, K., and Y. Suzuki, J. Lipid Res. 13: 687 (1972).
Kanfer, J.N., in “Methods in Enzymology”, Vol. 14, Edited by S.P. Colowick and N.O. Kaplan, Academic Press, 1969, p. 660.
Dubois, M., K.A. Gillis, J.K. Hamilton, P.A. Reebers and F. Smith, Anal. Chem. 28: 350 (1956).
Folch, J., M. Lees and G.H. Sloane-Stanley, J. Biol. Chem. 226: 497 (1957).
Moore, S., and K.P. Link, Ibid. 133: 293 (1940).
Kuhn, R., and H. Wiegandt, Chem. Ber. 96: 866 (1963).
Svennerholm, L., J. Lipid Res. 5: 145 (1964).
Kanfer, J.N., and M. Williams, Chem. Phys. Lipids 6: 121 (1971).
Petrovich, G., R.A. Mumford and J.N. Kanfer, Ibid. Chem. Phys. Lipids.in press.
Kanfer, J.N., J. Biol. Chem. 240: 609 (1965).
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Mumford, R.A., Raghavan, S.S., Rhoads, D.B. et al. Radiochemical Decomposition of lactosyl-6-[3H]-ceramide. Lipids 8, 238–240 (1973). https://doi.org/10.1007/BF02544643
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DOI: https://doi.org/10.1007/BF02544643