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Nature of fecal sterols and intestinal bacterial flora

  • Published:
Lipids

Abstract

Sterol excretion in the spontaneously atherosclerosis-susceptible White Carneau (WC) pigeon, the Silver King (SK) pigeon and the Show Racer (SR) pigeon was studied by thin layer chromatography (TLC), argentation TLC and gas liquid chromatography. Unlike man and the chicken, these pigeons excreted no coprostanol or coprostanone derivatives of sterols. Moreover incubation of14C-labeled cholesterol with pigeon feces indicated that, also unlike man and the chicken, these pigeons are unable to convert it to coprostanol. Bacteriologic examination revealed the absence of gram-negative anaerobic flora and of members of the genusBifidobacterium in both the WC and SR pigeons. On the other hand, one of the two SK pigeons examined showed evidence of the presence of bothBacteroids fragilis andB. bifidum in the upper intestinal tract. Although no qualitative experiments were performed, no unusual characteristics of the aerobic flora were noted in these pigeons. In addition, analysis of human stool specimens indicated a “normal” bowel flora. The flora of the intestinal tract of the chicken is similar to that of the human. Because of this similarity, it appears that differences in environment (living conditions, diets) between the human and the chicken are of little consequence. The results obtained in this study suggest the possibility that the anaerobic gram-negative flora and sponsible, at least in part, for the chemical conversion of cholesterol to coprostanol.

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Authors and Affiliations

  1. Mayo Clinic and Mayo Foundation, Rochester, Minnesota

    W. J. Martin, M. T. Ravi Subbiah, B. A. Kottke, C. C. Birk & M. C. Naylor

Authors
  1. W. J. Martin
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  2. M. T. Ravi Subbiah
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  3. B. A. Kottke
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  4. C. C. Birk
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  5. M. C. Naylor
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Additional information

The following nomenclature has been used for the steroids referred to in this paper: cholesterol, cholest-Δ5-en-3β-ol; coprostanol, 5β-cholestan-3β-ol; campesterol, 24-methylcholest-Δ5-en-3βol; stigmasterol, 24-ethylcholest-Δ5,22-dien-3β-ol; β-sitosterol, 24-ethylcholest-Δ5-en-3β-ol; coprocampestanol, 24-methyl-5β-cholestan-3β-ol; coprostigmastenol, 24-ethyl-5β-cholest-Δ22-en-3β-ol; coprostigmastanol, 24-ethyl-5β-cholestan-3β-ol; coprostanone, 5β-cholestan-3-one; campestanone, 24-methyl-5β-cholestan-3-one; stigmastenone, 24-ethyl-5β-cholest-Δ22-en-3-one; and β-sitostanone, 24-ethyl-5β-cholestan-3-one.

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Martin, W.J., Ravi Subbiah, M.T., Kottke, B.A. et al. Nature of fecal sterols and intestinal bacterial flora. Lipids 8, 208–215 (1973). https://doi.org/10.1007/BF02544637

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  • DOI: https://doi.org/10.1007/BF02544637

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