Abstract
We investigated the reaction of butyraldehyde and benzylamine and analyzed the products to identify the components that produce protein cross-linking in the reaction of butyraldehyde and proteins. When the mixtures of butyraldehyde and benzylamine were incubated at pH 7 and 37°C for 48 hr, many reaction products other than 2-ethyl-2-hexenal and Schiff bases of butyraldehyde and 2-ethyl-2-hexenal were produced. Fluorescent substance(s) were formed only in the presence of dissolved oxygen in the reaction mixture. Three new nonflourescent products—d,e andf—were isolated, and their structures are suggested to be 2,9-dibenzyl-4,6,8-triethyl-7-propyl-2,9-diazabicyclo[3,3,1] nona-3-ene (d), 1-phenyl-2-benzyl- 4,5,7-triethyl-6-propyl-1H,2H,3H,5H,6H,7H,8H-pyrido[1,2-clpyrimidine (e) and 1-phenyl-2-benzyl-4,5,7-triethyl-6-propyl-1H,2H,4aH,5H,6H,7H,8H-pyrido[1,2-c]pyrimidine (f). Formation of these compounds suggested that the protein, cross-linking with butyraldehyde is caused by the tretrameric dialdehyde formed by repeated aldol condensation and Michael reaction of butyraldehyde.
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Abbreviations
- NMR:
-
nuclear magnetic resonance
- GC:
-
gas chromatography
- GC-MS:
-
gas chromatography-mass spectrometry
- HPLC:
-
high pressure liquid chromatography
References
Esterbauer, H. (1982) inFree Radicals, Lipid Peroxidation and Cancer (McBrien, C.D.E., and Slater, T.F., eds.), pp. 101–128, Academic Press, New York.
Frankel, E.N. (1982)Progr. Lipid Res. 22, 1–33.
Jain, S.K., and Hochstein, P. (1980)Biochem. Biophys. Res. Commun. 92, 247–254.
Hochstein, P., and Jain, S.K. (1981)Fed. Proc. 40, 183–188.
Koster, J.F., and Slee, R.G. (1983)Biochim. Biophys. Acta 752, 233–239.
Koster, J.F., Slee, R.G., Rutten-Van Beysterveld, C.C.M., and Montfoort, A. (1983)Biochim. Biophys. Acta 754, 238–242.
Bouzas, J.O., and Karel, H. (1984)J. Agric. Food Chem. 32, 1387–1392.
Beppu, M., Murakami, K., and Kikugawa, K. (1986)Chem. Pharm. Bull. 34, 781–788.
Beppu, M., Murakami, K., and Kikugawa, K. (1987)Biochim. Biophys. Acta 897, 169–179.
Tappel, A.L. (1980) inFree Radicals in Biology (Pryor, A.W., ed.), Vol. IV, pp. 1–47, Academic Press, New York.
Kikugawa, K. (1986)Adv. Free Radical Biol. Med. 2, 389–417.
Kikugawa, K., and Beppu, M. (1987)Chem. Phys. Lipids 44, 277–296.
Csallany, A.S., Guan, M.D., Manwaring, J.D., and Addis, P.B. (1984)Anal. Biochem. 142, 277–283.
Kikugawa, K., Kato, T., and Iwata, A. (1988)Anal. Biochem. 174, 512–521.
Kikugawa, K., Takayanagi, K., and Watanabe, S. (1985)Chem. Pharm. Bull. 33, 5437–5444.
Kikugawa, K., Iwata, A., and Beppu, M. (1988)Chem. Parm. Bull. 36, 685–692.
Esterbauer, H., Koller, E., Slee, K.G., and Koster, J.F. (1986)Biochem. J. 239, 405–409.
Jurgens, G., Lang, J., and Esterbauer, H. (1986)Biochim. Biophys. Acta 875, 103–114.
Kikugawa, K., and Sawamura, A. (1987)J. Am. Oil Chem. Soc. 64, 1156–1162.
Nielsen, A.T. (1957)J. Am. Chem. Soc. 79, 2518–2524.
Morishima, I., and Yoshikawa, K. (1975)J. Am. Chem. Soc. 97, 2950–2956.
Buege, J.A., and Aust, S.D. (1978)Methods, Enzymol. 52, 302–310.
Kosugi, H., Kato, T., and Kikugawa, K. (1988)Lipids 23, 1024–1031.
Kosugi, H., and Kikugawa, K. (1989)Free Rad. Biol. Med. 7, 205–207.
Gains, K.C., Salhany J.M., Tuma, D.J., and Sorrell, M.F. (1977)FEBS Lett. 75, 115–119.
Donohue, T.M., Jr., Tuma, D.J., and Sorrell, M.F. (1983)Arch. Biochem. Biophys. 220, 239–246.
Donohue, T.M., Jr., Tuma, D.J., and Sorrell, M.F. (1983)Lab. Invest. 49, 226–229.
Tuma, D.J., and Sorrell, M.F. (1985) inAldehyde Adducts in Alcoholism (Collins, M.A., ed.) pp. 3–17, Alan R. Liss, Inc., New York.
Stevens, V.J., Fantl W.J., Newman, C.B., Sims, R.V., Cerami, A., and Peterson, C.M. (1981)J. Clin. Invest. 67, 361–369.
George, R.C.S., and Hoberman, H.D. (1986)J. Biol. Chem., 261, 6811–6821.
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Kikugawa, K., Kato, T. & Iwata, A. A tetrameric dialdehyde formed in the reaction of butyral dehyde and benzylamine: A possible intermediary component for protein cross-linking induced by lipid oxidation. Lipids 24, 962–969 (1989). https://doi.org/10.1007/BF02544542
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DOI: https://doi.org/10.1007/BF02544542