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Characterization of porcine omental lipids

  • Published:
Lipids

Abstract

Porcine omental lipid extracts were fractionated and the major lipid components characterized. Approximately 97% of the chloroform/methanol extract consisted of triglycerides containing primarily 16∶0, 18∶0, 18∶1, and 18∶2 fatty acids. Small quantities of free fatty acids, cholesterol, di- and monoglycerides were also detected. The phospholipid fraction, obtained by solvent partition and Unisil column chromatography and characterized by high performance liquid chromatography (HPLC) and HPLC-mass spectrometry, was found to consist primarily of phosphatidylcholine, sphingomyelin, phosphatidylethanolamine, phosphatidylserine, and phosphatidylinositol. The neutral glycolipids, isolated by solvent partition and Unisil column chromatography and identified by HPTLC and HPLC, were found to consist primarily of di-, tri-and tetraosylceramides. The complex glycolipid fraction, obtained from Folch upper phase solvent partition and characterized by HPTLC and immunoblotting, was found to consist primarily of ganglio-, globo-, and neolacto- neutral glycolipids and ganglio-, globo-, neolacto- and fucosylated gangliosides.

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Abbreviations

GlcCer:

glucosylceramide

GalCer:

galactosylceramide

LacCer:

lactosylceramide

GaOse2Cer:

galabiglycosylceramide, Gal(α1-4)Gal(β1−1)Cer

GbOse3Cer:

globotriglycosylceramide, Gal(α1-4)Gal(β1-4)Glc(β1-1)Cer

GbOse4Cer:

globotetraglycosylceramide, Ga1NAc(β1-3)Gal(α1-4)Gal(β1-4)Cer

GalNAcGbOse4Cer:

globotetraglycosylceramide, Ga1NAc(β1-3)Gal(α1-4)Gal(β1-4)Glc(β1-1)Cer

GalNAcGbOse4Cer:

Forssman glycolipid

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Authors and Affiliations

  1. Biochemistry Department, Eunice Kennedy Shriver Center, 200 Trapelo Rd., 02254, Waltham, MA

    Robert H. McCluer, James E. Evans & Marcia A. Williams

  2. Angio-Medical Corporation, at Shriver Center, 100 Beaver Street, 02154, Waltham, MA

    Ahmad Reza Kamarei

Authors
  1. Robert H. McCluer
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  2. James E. Evans
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  3. Ahmad Reza Kamarei
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  4. Marcia A. Williams
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McCluer, R.H., Evans, J.E., Kamarei, A.R. et al. Characterization of porcine omental lipids. Lipids 24, 951–956 (1989). https://doi.org/10.1007/BF02544540

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  • DOI: https://doi.org/10.1007/BF02544540

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