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LCAT inhibitors interfere with the enzymatic determination of cholesterol and triglycerides

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Lipids

Abstract

5,5-Dithiobis-(2-nitrobenzoic acid) (DTNB) andp-chloromercuriphenylsulfonic acid (PCMPS) are well-known sulfhydryl inhibitors that are used to inhibit lecithin: cholesterol acyltransferase (LCAT). They were each found to interfere with the enzymatic assays of cholesterol and triglycerides. DTNB falsely reduced the measured plasma cholesterol content, and falsely increased triglyceride readings. The inteference with the triglyceride assay could be largely prevented by blanking for glycerol. PCMPS had only a slight effect on the cholesterol assay, but falsely lowered the triglyceride readings to a great extent, even with glycerol-blanking. Thus, these inhibitors should be avoided when plasma samples are to be enzymatically analyzed for cholesterol or triglycerides.

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Abbreviations

DTNB:

5,5-dithiobis-(2-nitrobenzoic acid)

PCMPS:

p-chloromercuriphenylsulfonic acid

LCAT:

lecithin:cholesterol acyltransferase

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Authors and Affiliations

  1. Lipid and Arteriosclerosis Prevention Clinic, Division of Clinical Pharmacology, Department of Medicine, University of Kansas Medical Center, 66103, Kansas City, KS

    William S. Harris & Alan Rayford

Authors
  1. William S. Harris
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  2. Alan Rayford
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Harris, W.S., Rayford, A. LCAT inhibitors interfere with the enzymatic determination of cholesterol and triglycerides. Lipids 25, 341–343 (1990). https://doi.org/10.1007/BF02544344

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  • DOI: https://doi.org/10.1007/BF02544344

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