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Effect of dietary fat on the lipid composition and utilization of short-chain fatty acids by rat colonocytes

  • Published:
Lipids

Abstract

The objective of the present studies was to examine the effect of dietary fat on the lipid composition of rat colonocytes and their utilization of short-chain fatty acids (SCFA). Rats were fed 14% beef fat, fish oil or safflower oil plus 2% corn oil in a semi-synthetic base diet for 4 wk. Colonocytes were isolated and their lipid composition was examined. Feeding beef fat and fish oil resulted in an increase in monounsaturated fatty acids and a reduction in ω-6 fatty acids. Feeding fish oil resulted in an enrichment with ω-3 fatty acids. These was no dietary influence on the amount of either cholesterol or phospholipids of colonocytes. Fish oil feeding resulted in significant increase in colonocyte free fatty acids (FFA) as compared to other diets. Dietary fat was found to have no effect on SCFA utilization by colonocytes. Colonocytes were found to utilize SCFA in the order of butyrate ≥acetate ≥propionate. The presence of acetate and propionate in the medium had no effect on the rate of butyrate utilization.

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Abbreviations

SCFA:

short-chain fatty acids

PBS:

phosphate buffer saline

BSA:

bovine serum albumin

FFA:

free fatty acids

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Authors and Affiliations

  1. Nutrition Program, State University of New York at Buffalo, 323 Kimball Tower, 14214, Buffalo, NY

    Atif B. Awad, Sandra L. Ferger & Carol S. Fink

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  1. Atif B. Awad
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  2. Sandra L. Ferger
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  3. Carol S. Fink
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Awad, A.B., Ferger, S.L. & Fink, C.S. Effect of dietary fat on the lipid composition and utilization of short-chain fatty acids by rat colonocytes. Lipids 25, 316–320 (1990). https://doi.org/10.1007/BF02544340

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  • DOI: https://doi.org/10.1007/BF02544340

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