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Phospholipase a activity of cultured rat ventricular myocyte is affected by the nature of cellular polyunsaturated fatty acids

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Lipids

Abstract

Fatty acid composition of membrane phospholipids of cultured cardiomycotes can be modified by the type of polyunsaturated fatty acids (n−3 or n−6 PUFA) constituting the culture medium. In this study, we investigated the effect of fatty acid modification on the activities of the key enzymes involved in the deacylation-reacylation cycle of membrane phospholipids.

Results showed that cardiomyocytes grown in the presence of n−6 PUFA exhibited a higher specific alkaline phospholipase A (mainly A2) activity (+34%) and a moderately lower lysophospholipase activity(−17%) than when incubated with n−3 PUFA. AcylCoA:lysophosphatidylcholine acyltransferase, acid lysosomal phospholipase A1 and acylCoA synthetase activities were not significantly altered by changes in cellular PUFA composition. It was demonstrated that the differences between phospholipase A activities of the two types of cultured cells were linked neither to a differential leakage of enzyme nor to oxidative injury to the enzyme through blockage of essential sulfhydryl groups. One likely explanation is that the PUFA-induced changes in membrane composition alter membrane physical properties which, in turn, affect membrane-bound phospholipase A activity. Possible beneficial effects of the n−3 PUFA-induced changes on membrane stability are discussed.

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Abbreviations

PUFA:

polyunsaturated fatty acids

PC:

phosphatidylcholines

PE:

phosphatidylethanolamines

SM3:

n−3 PUFA-enriched synthetic media

SM6:

n−6 PUFA-enriched synthetic media

ACLAT:

acylCoA:lysophosphatidylcholine acyltransferase

BME:

betamercaptoethanol

PI:

phosphatidylinositol

DPG:

diphosphatidylglycerol

LPC:

lysophosphatidylcholine

LPE:

lysophosphatidylethanolamine

SPH:

sphingomyelin

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Authors and Affiliations

  1. Unite 130, Institut National de la Sante et de la Recherche Medicale, 18 Avenue Mozart, 13009, Marseille, France

    Gilles Nalbone, Jeannie Leonardi, Elise Termine & Huguette Lafont

  2. Institute National de la Recherche Agronomique, 17 rue Sully, 21034, Dijon, France

    Alain Grynberg & Annick Chevalier

Authors
  1. Gilles Nalbone
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  2. Alain Grynberg
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  3. Annick Chevalier
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  4. Jeannie Leonardi
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  5. Elise Termine
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  6. Huguette Lafont
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Nalbone, G., Grynberg, A., Chevalier, A. et al. Phospholipase a activity of cultured rat ventricular myocyte is affected by the nature of cellular polyunsaturated fatty acids. Lipids 25, 301–306 (1990). https://doi.org/10.1007/BF02544337

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  • DOI: https://doi.org/10.1007/BF02544337

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