Abstract
We studied the actions of oxysterols on fatty acid distribution and lipid synthesis in cultured bovine aortic smooth muscle cells. Cultures were labeled with [1-14C] arachidonate or [1-14C]oleate. During a 24-hr incubation, 25-or 22R-hydroxycholesterol enhanced the incorporation of label into triglycerides, concomitant with a reduction in the labeling of phospholipids. Cholestantriol or 20-hydroxycholesterol had the opposite effects. They caused a higher incorporation of radiolabel into phospholipids and a reduction of labeling of triacylglycerols. Similar changes were seen in cells labeled with [1-14C]acetate. Therefore, we conclude that oxysterols can promote changes in the distribution of fatty acids between neutral lipids and phospholipids through mechanisms that still need to be clarified.
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Abbreviations
- 20-Hydroxycholesterol:
-
cholest-5-en-3β,20α-diol
- 22R-hydroxycholesterol:
-
cholest-5-en-3β,22(R)-diol
- 25-hydroxycholesterol:
-
cholest-5-en-3β,25-diol
- cholestantriol:
-
cholestan-3β,5α,6β-triol
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Seillan, C. Oxysterol mediated changes in fatty acid distribution and lipid synthesis in cultured bovine aortic smooth muscle cells. Lipids 25, 172–176 (1990). https://doi.org/10.1007/BF02544334
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DOI: https://doi.org/10.1007/BF02544334