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Albumin-bound docosahexaenoic acid and collagen-induced human platelet reactivity

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Lipids

Abstract

Anin vitro system designed to mimic the effect of various plasma nonesterified (polyunsaturated) fatty acids on platelet function and metabolism was employed. Human platelet aggregation induced by submaximal (1.8 μg/ml) collagen stimulation was significantly inhibited by 2 min preincubation with 20 μM albumin-bound docosahexaenoic acid (22∶6n−3) (DHA), but not by the other fatty acids tested. [3H]Phosphatidic acid (PA) formation, an indicator of phospholipase C activation following platelet stimulation, was moderately inhibited by eicosapentaenoic acid (20∶5n−3), 11,14,17-eicosatrienoic acid (20∶3n−3), dihomo-γ-linolenic acid (20∶3n−6), as well as DHA, but not by arachidonic acid (20∶4n−6); this inhibition of phospholipase C activation could not explain the differential effect of DHA on platelet aggregation. The decreased production of thromboxane A2 (TxA2), as assessed by [3H]12-hydroxy-5,8,10-heptadecatrienoic acid (HHT) formation, may account for the inhibition of collagen-induced aggregation by 20 μM DHA. Surprisingly, preincubation with 40 μM albumin-bound DHA, even though resulting in greater inhibition of collagen-induced aggregation, had less impact on HHT formation. A small but significant increase in [3H]prostaglandin D2 (PGD2) levels following 3-min collagen stimulation may have contributed to the greater antiaggregatory effect of 40 μM DHA. It is concluded that increased plasma nonesterified DHA may contribute to the dampened platelet activation and altered metabolism following fish oil supplementation of the diet.

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Abbreviations

AA:

arachidonic acid

DG:

diacylglycerol

DHA:

docosahexaenoic acid

EPA:

eicosapentaenoic acid

FFA:

free fatty acids

HETE:

hydroxyeicosatetraenoic acid

HHT:

hydroxy-heptadecatrienoic acid

TP3 :

inositol trisphosphate

NEFA:

nonesterified fatty acid

PA:

phosphatidic acid

PGD2 :

prostaglandin D2

TxA2 :

thromboxane A2

TxB2 :

thromboxane B2

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Authors and Affiliations

  1. Department of Nutritional Sciences, University of Guelph, N1G 2W1, Guelph, Ontario, Canada

    Douglas C. Gaudette & Bruce J. Holub

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  1. Douglas C. Gaudette
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  2. Bruce J. Holub
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Gaudette, D.C., Holub, B.J. Albumin-bound docosahexaenoic acid and collagen-induced human platelet reactivity. Lipids 25, 166–169 (1990). https://doi.org/10.1007/BF02544332

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  • DOI: https://doi.org/10.1007/BF02544332

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