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A method for the quantitative analysis of molecular species of alkylacylglycerol and diacylglycerol

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Lipids

Abstract

We describe a method for the quantitative analysis of molecular species of diacylglycerol and alkylacylglycerol as their diradylglycerobenzoate derivatives. Synthetic internal standards were used to provide quantitative determinations of the low levels of diacylglycerol and alkyl-acylglycerol and their individual molecular species in cultured cells. Diradylglycerols were isolated by thin-layer chromatography (TLC), converted to their benzoate derivatives and separated into subclasses by TLC. The molecular species of each subclass were analyzed by reversed-phase high performance liquid chromatography. Thirty-six species of diglyceride-type molecules were identified in Madin-Darby canine kidney cells. These cells were shown to contain 7.88 nmoles of diacylglycerol and 3.97 nmoles of alkylacylglycerol per μmole of phospholipid. Both subclasses contain predominantly monoenoic and saturated species. This technique should be valuable for studies examining the origin and metabolism of these important intracellular mediators.

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Abbreviations

AAG:

alkylacylglycerol

DAG:

diacylglycerol

GLC:

gas liquid chromatography

HPLC:

high performance liquid chromatography

MDCK:

Madin-Darby canine kidney

TLC:

thin-layer chromatography

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Authors and Affiliations

  1. Department of Biochemistry, James H. Quillen College of Medicine, East Tennessee State University, 37614, Johnson City, Tennessee

    Thomas R. Warne & Mitchell Robinson

Authors
  1. Thomas R. Warne
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  2. Mitchell Robinson
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Warne, T.R., Robinson, M. A method for the quantitative analysis of molecular species of alkylacylglycerol and diacylglycerol. Lipids 25, 748–752 (1990). https://doi.org/10.1007/BF02544045

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  • DOI: https://doi.org/10.1007/BF02544045

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