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Intermembrane cholesterol transfer: Role of sterol carrier proteins and phosphatidylserine

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Lipids

Abstract

The effect of phosphatidylserine and sterol carrier proteins on cholesterol exchange was determined using an assay not requiring separation of donor and acceptor membrane vesicles. Sterol carrier protein-2 (SCP2, also called nonspecific lipid transfer protein), but not fatty acid binding protein (FABP, also called sterol carrier protein), enhanced the initial rate of sterol exchange between neutral zwitterionic phosphatidylcholine small unilamellar vesicles (SUV) 2.3-fold. Phosphatidylserine at 10 mol% increased the initial rate of spontaneous and of SCP2-mediated (but not FABP-mediated) sterol exchange by 22% and 44-fold, respectively. The SCP2 potentiation of sterol transfer was dependent on SCP2 concentration and on phosphatidylserine concentration. The SCP2-mediated sterol transfer was inhibited by a variety of cations including KCl, divalent metal ions, and neomycin. The data suggest that SCP2 increase in activity for sterol transfer may be partly ascribed to charge on the phospholipid.

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Abbreviations

DHE:

dehydroergosterol

FABP:

fatty acid binding protein

HPLC:

high performance liquid chromatography

PC:

1-palmitoyl-2-oleoyl phosphatidylcholine

PS:

phosphatidylserine

SCP2 :

sterol carrier protein

SUV:

small unilamellar vesicles

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Authors and Affiliations

  1. Division of Pharmacology and Medicinal Chemistry, Department of Pharmacology and Cell Biophysics, University of Cincinnati Medical Center, 45267-0004, Cincinnati, Ohio

    Friedhelm Schroeder, Peter Butko & Ivan Hapala

  2. Department of Biochemistry, School of Medicine, University of New Mexico, 87131, Albuquerque, New Mexico

    Terrence J. Scallen

Authors
  1. Friedhelm Schroeder
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  2. Peter Butko
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  3. Ivan Hapala
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  4. Terrence J. Scallen
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Additional information

A portion of this work was presented as an abstract: Nemecz, G., Butko, P., and Schroeder, f. (1989)Biophysical Journal 55, 137a.

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Schroeder, F., Butko, P., Hapala, I. et al. Intermembrane cholesterol transfer: Role of sterol carrier proteins and phosphatidylserine. Lipids 25, 669–674 (1990). https://doi.org/10.1007/BF02544032

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  • DOI: https://doi.org/10.1007/BF02544032

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