Abstract
Food restriction was found to impair the incorporation of [1-14C]palmitate into myelin membrane lipids of developing rat brain. An attempt was made to determine whether this phenomenon is due to differences in the rate of utilization of the labelled precursor or to its enhanced degradationvia β-oxidative pathways. Undernutrition in pups was imposed by food restriction during gestation and lactation. β-Oxidation by brain region homogenates using [1-14C]palmitate was monitored at days 7, 14 and 21 of postnatal age. There was a significant increase in β-oxidation in the brain regions of undernourished pups, with the cerebrum and cerebellum being more affected than the brain stem. Because developing brain possesses the enzymic potential to utilize ketone bodies, the data may indicate increased usage of palmitate as an energy source in the developing brain of undernourished animals.
Similar content being viewed by others
Abbreviations
- EDTA:
-
ethylenediaminetetraacetic acid
- FID:
-
flame ionization detector
- TLC:
-
thin layer chromatography
- Tris:
-
(hydroxymethyl)aminoethane
- UV:
-
ultraviolet light
References
Wiggins, R.C. (1982)Brain Res. Rev. 4, 151–175.
Reddy, P.V., Das, A., and Sastry, P.S. (1979)Brain Res. 161, 227–235.
padmini, S. (1989)Biochemical Studies on Myelinogenesis in the Developing Brain in Relation to Nutritional Stress, Ph.D. Thesis, Osmania University, Hyderabad, India, pp. 52–56.
Edmond, J., Robbins, R.A., Bergstrom, J.D., Cole, R.A., and de Vellis, J. (1987)J. Neurosci. Res. 18, 551–561.
Williamson, D.H. (1982) inThe Biochemical Development of the Fetus and Neonate (Jones, C.T., ed.) pp. 621–650, Elsevier Biomedical Press, Amsterdam.
Spitzer, J.J. (1973)Physiologist 16, 55–68.
Menon, N.K., Dhopeshwarkar, G.A., and Mead, J.F. (1984)Lipids 19, 122–126.
Folch, J., Lees, M., and Sloane-Stanley, G.H. (1957)J. Biol. Chem. 226, 497–509.
Kishimoto, Y., and Hoshi, M. (1972) inMethods of Neurochemistry (Fried, R., ed.) pp. 81–84, Marcel Dekker, New York.
Winick, M. (1976) inMalnutrition and Brain Develoment, Oxford University Press, New York.
Edmond, J., Auestad, N., Robbins, R.A., and Bergstrom, J.D. (1985)Fed. Proc. 44, 2359–2364.
Bhasin, S., and Shambaugh, G.E. III (1982)Am. J. Physiol. 243, E234–239.
Author information
Authors and Affiliations
About this article
Cite this article
Padmini, S., Rao, P.S. Enhanced β-oxidative utilization of [1-14C]Palmitate during active myelinogenesis in developing rat brain under nutritional stress. Lipids 26, 83–85 (1991). https://doi.org/10.1007/BF02544031
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF02544031