Bacteriedoodende stoffen in weefselculturen

Summary

• 1. Author discribes a method which renders the technique of tissue culture, as planned by de Haan (Groningen) better fit to immunization studies in vitro; also exposes why this principle of tissue culture is particularly adapted for this special field of research.

• 2. Wright's slide cell technique has been adopted for the study of bacteria-killing substances in tissue cultures.

• 3. A series of observations confirms in the main the results of non-specific immunity in rabbits as found by Wolff and Ras. The tissues prove to be sensitive to “vaccination” with a number of various substances, after which they give a bacteria-killing power to the culturefluid.

• 4. It came out that two kinds of bacteria-killing substances are formed

• A. Primary bacteriacides, probably by decay of leucocytes.

• B. Vaccination bacteriacides formed by syncytial cel-elements as a reaction to non-specific stimulation

• 5. The tissues are to be accustomed to a definite stimulation but after this habituation-test they remain sensitive to vaccination of some other kind.

• 6. The disappearance of bacteria-killing substances is not consequence of their chemical instability, but they in turn are destroyed by the living cells which had produced them.