Summary
The synergism between chloramphenicol and polymyxin B sulphate, earlier noticed using a replica technique, has been reexamined applying quantitative methods. Vital count experiments in suspensions ofSalmonella typhimurium in broth have revealed that addition of bacteriostatic concentrations of chloramphenicol in a range of 5–100 μg/ml to bacteriostatic concentrations of polymyxin (0.1–0.9 μg/ml) produce a strong bactericidal effect (synergism). Combinations of bactericidal concentrations of polymyxin (1 μg/ml and higher) with chloramphenicol also exert a very high degree of bactericidal activity, though no more than with polymyxin alone (no synergism).
Chloramphenicol therapy sustained with polymyxin, may be of therapeutic value in the eradication of stubborn enteralSalmonella infections.
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References
Chabbert, Y. 1953. Ann. Inst. Pasteur84 545.
Elek, S. D., Hilson, G. R. F. andJewell, P. 1953. Br. Med. J. II, 1298.
Fairbrother, R. W. andSouthall, J. E. 1957. Lancet II, 974.
Gaikhorst, G. 1955. Thesis, Utrecht.
Jawetz, E. andGunnison, J. B. 1952. Antib. Chemoth.2, 243.
Julius, H. W. andGaikhorst, G. 1958. Antib. Chemoth.8, 287.
Lederberg, J. andLederberg, E. M. 1952. J. Bact.63, 399.
Manten, A. andde Nooy, J. A. 1956. Antonie van Leeuwenhoek22, 231.
Manten, A. 1954. Antib. Chemoth.4, 1228.
Manten, A. 1956. Antib. Chemoth.6, 480.
Oskam, H. J. 1956. Act. Med. Scand.155, 335.
Weil, A. J. andKotsevalov, O. 1956. Zentrbl. Bakt.165, 459.
Zwart Voorspuy, A. J. andBokma, L. H. 1958. Antib. Chemoth.8, 171.
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With the technical assistance of MissM. J. Wisse.
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Manten, A., de Nooy, J.A. Some further observations on synergism between chloramphenicol and polymyxin B in relation toSalmonella bacteria. Antonie van Leeuwenhoek 25, 183–187 (1959). https://doi.org/10.1007/BF02542845
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DOI: https://doi.org/10.1007/BF02542845