Summary
From the oxidation of malate and citrate (instead of isocitrate) by livingE. coli cells no formation of glyoxylate could be observed. On the other hand glyoxylate was always formed in the presence of acetate or glycolate. This result is further proof that glyoxylate is a direct intermediate of the oxidation of acetate and glycolate according to the monocarboxylic acid scheme.
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Bolcato, V. Glyoxylate intermediate of the direct oxidation of acetate and glycolate byE. coli . Antonie van Leeuwenhoek 25, 179–182 (1959). https://doi.org/10.1007/BF02542844
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DOI: https://doi.org/10.1007/BF02542844