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Development of a pilot plant process for the preparation of a soy trypsin inhibitor concentrate

  • Technical
  • Published:
Journal of the American Oil Chemists’ Society

Abstract

A pilot-plant procedure was developed to prepare a soy trypsin inhibitor (TI) concentrate in sufficient quantities to support a lifetime (2-yr) feeding trial in which diets containing varying amounts of TI would be fed to rats to assess the physiological effects on the pancreas and other organs. Starting with water dispersions of commercial defatted soy flour, separation of TI (MW<21,500) from non-TI protein (MW 180,000–350,000) by virtue of their MW difference was attempted using ultrafiltration techniques but was not successful. However, good separation was obtained when selective acid precipitation coupled with “salting in” of the TI with 0.1 N sodium chloride was employed. Low MW components were separated successfully by ultrafiltration using a 1,000 MW cutoff membrane. The final soy TI concentrate obtained by freeze drying exhibited a 9-fold increase in TI activity.

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Authors and Affiliations

  1. U.S. Department of Agriculture, Northern Regional Research Center, Agricultural Research Service, 61604, Peoria, Illinois

    E. C. Baker & J. J. Rackis

Authors
  1. E. C. Baker
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  2. J. J. Rackis
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Baker, E.C., Rackis, J.J. Development of a pilot plant process for the preparation of a soy trypsin inhibitor concentrate. J Am Oil Chem Soc 62, 84–87 (1985). https://doi.org/10.1007/BF02541496

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  • DOI: https://doi.org/10.1007/BF02541496

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