Journal of the American Oil Chemists’ Society

, Volume 69, Issue 10, pp 986–991 | Cite as

Lipase-catalyzed transesterification of phosphatidylcholine at controlled water activity

  • Ingemar Svensson
  • Patrick Adlercreutz
  • Bo Mattiasson


The incorporation of a free fatty acid into thesn-1 position of phosphatidylcholine by lipase-catalyzed transesterification was investigated. The thermodynamic water activity of both the enzyme preparation and the substrate solution was adjusted to the same value prior to the reaction. The reaction rate increased with increasing water activity but the yield of modified phosphatidylcholine decreased due to hydrolysis. By using a large excess of the free fatty acid (heptadecanoic acid), the hydrolysis reaction was slowed down, so a higher yield was obtained at a given degree of incorporation. The best results were obtained withRhizopus arrhizus lipase immobilized by adsorption on a polypropylene support. With this preparation, a yield of 60% and nearly 50% incorporation of heptadecanoic acid (100% incorporation in thesn-1 position) was obtained at a water activity of 0.064. The enzyme preparation had good operational stability and position specificity. Little incorporation (<1%) was observed in thesn-2 position, when almost all the fatty acid in thesn-1 position was exchanged.

Key words

Lipase phosphatidylcholine transesterification water activity 


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. 1.
    Plückthun, A. and E.A. Dennis,Biochemistry 21:1743 (1982).CrossRefGoogle Scholar
  2. 2.
    Brockerhoff, H., P.C. Schmidt, J.W. Fong and L.J. Tirri,Lipids 11:421 (1976).CrossRefGoogle Scholar
  3. 3.
    Yoshimoto, T., M. Nakata, S. Yamaguchi, T. Funada, Y. Saito and Y. Inada,Biotechnol. Lett. 8:771 (1986).CrossRefGoogle Scholar
  4. 4.
    Yagi, T., T. Nakanishi, Y. Yoshizawa and F. Fukui,J. Ferment. Bioeng. 69:23 (1990).CrossRefGoogle Scholar
  5. 5.
    Yoichiro, T., and H. Setsuko,J. Am. Oil Chem. Soc. 68:848 (1991).Google Scholar
  6. 6.
    Svensson, I., P. Adlercreutz and B. Mattiasson,Appl. Microbiol. Biotechnol. 33:255 (1990).CrossRefGoogle Scholar
  7. 7.
    Pernas, P., L.J. Olivier, M.D. Legoy and G. Bereziat,Biochem. Biophys. Res. Commun. 168:644 (1990).CrossRefGoogle Scholar
  8. 8.
    Na, A., C. Eriksson, S.G. Eriksson, E. Österberg and K. Holmberg,J. Am. Oil Chem. Soc. 67:766 (1990).Google Scholar
  9. 9.
    Adlercreutz, P.,Eur. J. Biochem. 199:609 (1991).CrossRefGoogle Scholar

Copyright information

© AOCS Press 1992

Authors and Affiliations

  • Ingemar Svensson
    • 1
  • Patrick Adlercreutz
    • 1
  • Bo Mattiasson
    • 1
  1. 1.Department of Biotechnology, Chemical CenterUniversity of LundLundSweden

Personalised recommendations