Skip to main content
SpringerLink
Log in

Reversed-Phase high-performance liquid chromatographic analysis of triacylglycerol autoxidation products with ultraviolet and evaporative light-scattering detectors

  • Short Communications
  • Published:
Journal of the American Oil Chemists’ Society

Abstract

The oxidation products of triacylgycerol standards (trilinolenin, trilinolein and triolein) and a natural mixture of rapeseed oil triacylglycerols were analyzed with a reversed-phase high-performance liquid chromatographic method. The oxidation products were detected with ultraviolet (UV) and evaporative light-scattering detectors (ELSD). The chromatographic profiles obtained with these two detectors were similar for all samples except triolein. ELSD is a mass detector and can detect the oxidation products of triolein and other compounds that do not have conjugated dienes in their structure. The two detectors can be used in series. The sensitivity of ELSD approached that of the UV detector used. ELSD seems to be a good universal detector type for monitoring autoxidation products of edible oils.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

References

  1. Park, D.K., J. Terao and S. Matsushita,Agric. Biol. Chem. 45:2443 (1981).

    CAS  Google Scholar 

  2. Neff, W.E., E.N. Frankel and K. Miyashita,Lipids 25:33 (1990).

    CAS  Google Scholar 

  3. Frankel, E.N., W.E. Neff and K. Miyashita,Ibid. 25:40 (1990).

    CAS  Google Scholar 

  4. Miyashita, K., E.N. Frankel, W.E. Neff and R.A. Awl,Ibid. 25: 48 (1990).

    CAS  Google Scholar 

  5. Neff, W.E., E. Selke, T.L. Mounts, W. Rinsch, E.N. Frankel and M.A.M. Zeitoun,J. Am. Oil Chem. Soc. 69:111 (1992).

    CAS  Google Scholar 

  6. Lampi, A.-M., A. Hopia, P. Ekholm and V. Piironen,Lebensm. Wiss. Technol. 25:386 (1992).

    CAS  Google Scholar 

  7. Ueda, S., T. Hayashi and M. Namiki,Agric. Biol. Chem. 50:1 (1986).

    CAS  Google Scholar 

  8. Hopia, A.I., V.I. Piironen, P.E. Koivistoinen and L.E.T. Hyvónen,J. Am. Oil Chem. Soc. 69:772 (1992).

    CAS  Google Scholar 

  9. Christie, W.W., inAdvances in Lipid Methodology—One, edited by W.W., Christie, The Oily Press Ltd., Ayr, 1992, p. 240.

    Google Scholar 

  10. Hopia, A.I., and V.-M. Ollilainen,J. Liquid Chrom. 16:2469 (1993).

    CAS  Google Scholar 

  11. Park, D.K., J. Terao and S. Matsushita,Agric. Biol. Chem. 45:2071 (1981).

    CAS  Google Scholar 

  12. Chan, H.W.-S. and G. Levett,Lipids 12:99 (1977).

    Article  CAS  Google Scholar 

  13. Souci, S.W., W. Fachmann and H. Kraut,Food Composition and Nutrition Tables 1986/87, 3rd. edn., Wissenschaftliche Verlagsgesellschaft, 1986.

Download references

Author information

Authors and Affiliations

  1. Department of Applied Chemistry and Microbiology, University of Helsinki, P.O. Box 27, SF-00014, Finland

    Eija Viinanen & Anu Hopia

Authors
  1. Eija Viinanen
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Anu Hopia
    View author publications

    You can also search for this author in PubMed Google Scholar

About this article

Cite this article

Viinanen, E., Hopia, A. Reversed-Phase high-performance liquid chromatographic analysis of triacylglycerol autoxidation products with ultraviolet and evaporative light-scattering detectors. J Am Oil Chem Soc 71, 537–539 (1994). https://doi.org/10.1007/BF02540668

Download citation

  • Received:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF02540668

Key Words

Search

Navigation