Journal of the American Oil Chemists’ Society

, Volume 67, Issue 11, pp 757–760 | Cite as

HPLC analysis of phospholipids in crude oil for evaluation of soybean deterioration

  • T. L. Mounts
  • A. M. Nash


Damage to soybeans due to pre-harvest stress, storage, and export shipment has been related to an increase in the nonhydratable phospholipid content of crude oil. Phospholipids in crude soybean oil extracted from such distressed soybeans have been analyzed by gradient high-performance liquid chromatography. Crude oil was fractionated by solid phase extraction using sequential elution for recovery of phosphatides. High-performance liquid chromatography of the concentrated phospholipids was accomplished on a Lichrosorb Si-60 10 μ column, 250×4.6 mm with ultraviolet detection at 206 nm. A 20-min solvent gradient of 2-propanol/hexane/water (42∶56∶2, 51∶38∶11) gave retention profiles of phospholipid distribution (major subclasses) that changed with impact of stress applied to plant or seed. Soybeans stored at high moisture levels (16% and 20% moisture) for up to 28 days yielded oils having phosphorus contents which decreased in direct relationship to days of storage. Retention profiles were unusable for fractions isolated from oils with phosphorus content below 100 ppm. Data show that during progressive damage, the content of phosphatidylcholine and phosphatidylinositol decreased while the phosphatidic acid content increased.

Key Words

Chromatography crude soybean oil degumming enzymes high performance liquid chromatography oil quality phosphatidylcholine phosphatidylethanolamine phosphatidylinositol phosphatidylserine 


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. 1.
    Soybean Dig. 32:7 (1971).Google Scholar
  2. 2.
    Christensen, C.M., and H.H. Kaufmann, inSoybeans: Chemistry and Technology, edited by A.K. Smith, and S.J. Circle, Avi Publishing Co., Westport, CT, 1972, p. 294.Google Scholar
  3. 3.
    Paulsen, M.R., W.R. Nave, T.L. Mounts and L.E. Gray,Trans. Am. Soc. Agric. Eng. 24(6): 1583 (1981).Google Scholar
  4. 4.
    Mounts, T.L., G.R. List and A.J. Heakin,J. Am. Oil Chem. Soc. 56:883 (1979).Google Scholar
  5. 5.
    Robertson, J.A., W.H. Morrison III and D. Burdick,Ibid. 50:443 (1973).Google Scholar
  6. 6.
    Evans, C.D., G.R. List, R.E. Beal and L.T. Black,Ibid. 51:444 (1974).Google Scholar
  7. 7.
    Carr, R.A.,Ibid. 53:347 (1976).Google Scholar
  8. 8.
    Braae, B.Chem. Ind., 1158 (1958).Google Scholar
  9. 9.
    Nakayama, Y., K. Saio and M. Kito,Cereal Chem. 58:260 (1987).Google Scholar
  10. 10.
    Hvolby, A.,J. Am. Oil Chem. Soc. 48:503 (1971).Google Scholar
  11. 11.
    Frankel, E.N., A.M. Nash and J.M. Synder,Ibid. 64:987 (1987).Google Scholar
  12. 12.
    Nash, A.M., and E.N. Frankel,Ibid. 63:244 (1986).Google Scholar
  13. 13.
    Official and Tentative Methods of the American Oil Chemists' Society, 3rd edn., edited by R.O. Walker, AOCS, Champaign, IL, 1981.Google Scholar
  14. 14.
    Geurts van Kessel, W.S.M., W.M.A. Hax, R.A. Demel and J. de Gier,Biochim. Biophys. Acta, 486:524 (1977).Google Scholar
  15. 15.
    Hax, W.M.A., and W.S.M. Geurts van Kessel,J. Chromatogr. 142:735 (1977).CrossRefGoogle Scholar
  16. 16.
    Yandrasitz, J.R., G. Berry and S. Segal,Ibid. 225:319 (1981).Google Scholar
  17. 17.
    Rivnay B.,Ibid. 294:303 (1984).CrossRefGoogle Scholar
  18. 18.
    Dugan, L.L., P. Demediuk, C.E. Pendley II and L.A. Horrocks,Ibid., 378:317 (1986).Google Scholar
  19. 19.
    Ragan, J.E., and A.P. Handel,J. Am. Oil Chem. Soc. 62:1568 (1985).Google Scholar
  20. 20.
    Scholfield, C.R., inLecithins, edited by B.F. Szuhaj, and G.R. List, American Oil Chemists' Society, Champaign, IL, 1985, pp. 1–20.Google Scholar

Copyright information

© AOCS Press 1990

Authors and Affiliations

  • T. L. Mounts
    • 1
  • A. M. Nash
    • 1
  1. 1.Vegetable Oil Research, Northern Regional Research Center, Agricultural Research ServiceUSDAPeoriaUSA

Personalised recommendations